Experimental investigations of mastrevirus molecular biology and evolution

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dc.contributor.advisorRybicki, Eden_ZA
dc.contributor.advisorMartin, D Pen_ZA
dc.contributor.authorVan der Walt, Ericen_ZA
dc.date.accessioned2014-07-30T17:42:15Z
dc.date.available2014-07-30T17:42:15Z
dc.date.issued2008en_ZA
dc.descriptionIncludes abstract.
dc.descriptionIncludes bibliographical references (leaves 138-161).
dc.description.abstractThis dissertation describes three major sets of experiments, all of which involved the construction and use of various reciprocal chimaeric MSV constructs. First, chimaeric viruses were used in genetic complementation-type experiments to investigate the biological significance of interactions between the two virion-sense open reading frames (ORFs) of MSV, their products, and the rest of the genome. Six chimaeric MSV constructs were made by reciprocally exchanging the ORFs encoding movement protein (MP) and coat protein (CP) individually, and in pairs, between MSV-Kom and MSV-Set, which share just 78% overall nucleotide identity. Analysis of symptomatology and infection efficiency of chimaeras and wild-type parental viruses revealed evidence of functionally relevant specific interactions between MSV MP and CP.en_ZA
dc.identifier.apacitationVan der Walt, E. (2008). <i>Experimental investigations of mastrevirus molecular biology and evolution</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4346en_ZA
dc.identifier.chicagocitationVan der Walt, Eric. <i>"Experimental investigations of mastrevirus molecular biology and evolution."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008. http://hdl.handle.net/11427/4346en_ZA
dc.identifier.citationVan der Walt, E. 2008. Experimental investigations of mastrevirus molecular biology and evolution. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Van der Walt, Eric AB - This dissertation describes three major sets of experiments, all of which involved the construction and use of various reciprocal chimaeric MSV constructs. First, chimaeric viruses were used in genetic complementation-type experiments to investigate the biological significance of interactions between the two virion-sense open reading frames (ORFs) of MSV, their products, and the rest of the genome. Six chimaeric MSV constructs were made by reciprocally exchanging the ORFs encoding movement protein (MP) and coat protein (CP) individually, and in pairs, between MSV-Kom and MSV-Set, which share just 78% overall nucleotide identity. Analysis of symptomatology and infection efficiency of chimaeras and wild-type parental viruses revealed evidence of functionally relevant specific interactions between MSV MP and CP. DA - 2008 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - Experimental investigations of mastrevirus molecular biology and evolution TI - Experimental investigations of mastrevirus molecular biology and evolution UR - http://hdl.handle.net/11427/4346 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4346
dc.identifier.vancouvercitationVan der Walt E. Experimental investigations of mastrevirus molecular biology and evolution. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4346en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleExperimental investigations of mastrevirus molecular biology and evolutionen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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