Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases

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dc.contributor.advisorvon Holt, C
dc.contributor.advisorVon Holt, Claus
dc.contributor.authorSabbatini, G P
dc.contributor.authorSabbatini, G P
dc.date.accessioned2017-01-26T11:32:34Z
dc.date.available2017-01-26T11:32:34Z
dc.date.issued1992
dc.date.updated2016-11-22T10:49:31Z
dc.description.abstractThe postulated model for the insulin - stimulated induction of mRNA efflux (Purrello et al., 1983) is based on the demonstrated binding of insulin to intracellular membrane structures (see chapter 2, section 2.2.1), and the in vitro effect of insulin on nuclear envelope phosphorylation, NTPase activity, and mRNA efflux (see chapter 5, section 5.1). These independent observations have led to the development of a model for the direct induction by insulin, at the level of the nucleus, of mRNA efflux (figure 1.1). However, the specific intracellular insulin binding has been inf erred from kinetic or morphological studies which have not identified a discrete membrane - bound polypeptide(s) as an insulin docking molecule in situ (Goldfine, 1981). Also, the stimulation of NTPase activity has only been established by monitoring the level of general ATP hydrolysis of nuclear envelope fractions in the presence and absence of insulin (Purrello et al., 1983). The scope of this thesis has been to further the understanding of this mechanism by attempting to a) unequivocally identify a specific nuclear envelope - associated insulin docking polypeptide in situ and b) to demonstrate that insulin directly affects the ATP - binding of nuclear envelope ATP - binding proteins. The latter would demonstrate a primary effect of insulin i.e. the modulation of the ATP - binding capacity of identified NTPases / protein kinases (or their release from some inactive storage form), and not a general phenomenon such as elevated ATP.
dc.identifier.apacitationSabbatini, G. P., & Sabbatini, G. P. (1992). <i>Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/23361en_ZA
dc.identifier.chicagocitationSabbatini, G P, and G P Sabbatini. <i>"Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1992. http://hdl.handle.net/11427/23361en_ZA
dc.identifier.citationSabbatini, G., Sabbatini, G. 1992. Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Sabbatini, G P AU - Sabbatini, G P AB - The postulated model for the insulin - stimulated induction of mRNA efflux (Purrello et al., 1983) is based on the demonstrated binding of insulin to intracellular membrane structures (see chapter 2, section 2.2.1), and the in vitro effect of insulin on nuclear envelope phosphorylation, NTPase activity, and mRNA efflux (see chapter 5, section 5.1). These independent observations have led to the development of a model for the direct induction by insulin, at the level of the nucleus, of mRNA efflux (figure 1.1). However, the specific intracellular insulin binding has been inf erred from kinetic or morphological studies which have not identified a discrete membrane - bound polypeptide(s) as an insulin docking molecule in situ (Goldfine, 1981). Also, the stimulation of NTPase activity has only been established by monitoring the level of general ATP hydrolysis of nuclear envelope fractions in the presence and absence of insulin (Purrello et al., 1983). The scope of this thesis has been to further the understanding of this mechanism by attempting to a) unequivocally identify a specific nuclear envelope - associated insulin docking polypeptide in situ and b) to demonstrate that insulin directly affects the ATP - binding of nuclear envelope ATP - binding proteins. The latter would demonstrate a primary effect of insulin i.e. the modulation of the ATP - binding capacity of identified NTPases / protein kinases (or their release from some inactive storage form), and not a general phenomenon such as elevated ATP. DA - 1992 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1992 T1 - Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases TI - Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases UR - http://hdl.handle.net/11427/23361 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/23361
dc.identifier.vancouvercitationSabbatini GP, Sabbatini GP. Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1992 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/23361en_ZA
dc.language.isoeng
dc.language.isoeng
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biology
dc.titleRat liver nuclear envelope insulin binding and its effects on endogenous protein kinases
dc.titleRat liver nuclear envelope insulin binding and its effects on endogenous protein kinases
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhD
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearch
uct.type.resourceThesis
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