Molecular characterisation of selected gastrointestinal microbiota in South African HIV-positive patients during HAART

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dc.contributor.advisorAbratt, Valerie Roseen_ZA
dc.contributor.advisorReid, Sharon Jen_ZA
dc.contributor.authorDu Plessis, Sarah Janeen_ZA
dc.date.accessioned2014-12-28T14:49:57Z
dc.date.available2014-12-28T14:49:57Z
dc.date.issued2012en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractProgression of the HIV disease is characterised by a massive depletion of CD4+ T cells and it has been shown that patients living with a more advanced HIV infection have a higher risk of developing diarrhoea due to the disruption of the gastrointestinal microbiota caused by either the HIV-infection or the use of antibiotics and drugs such as highly active antiretroviral therapy (HAART). An imbalance in the microbial composition, attributable to a disturbed mucosal barrier, as well as increased permeability and inflammation caused by HIV, can influence the metabolic (carbohydrate fermentation) and protective functions provided by the microbiota. The effect of HIV on the intestinal microbiota has not been widely examined and those studies that have focused on HIV and the gastrointestinal tract, have investigated it mainly from a virological perspective. Consequently, the aim of the study was to ascertain whether the diversity and/or abundance of the endogenous intestinal microbiota of South African HIV-positive patients was disrupted on account of HIV within the gastrointestinal tract. An additional aim was to determine whether the administration of HAART affected the microbiota during a 6 month longitudinal study. The diversity of the intestinal microbial composition was characterised with respect to the total bacteria, Bifidobacterium and Lactobacillus species using PCR-DGGE. qPCR was used to determine the abundance of total bacteria, Bifidobacterium, Lactobacillus, Escherichia coli, the Bacteroides/Prevotella, Clostridium coccoides and Clostridium leptum groups. ... In addition, three potential intestinal pathogens (Clostridium difficile, Campylobacter jejuni and Salmonella enterica) were monitored by qPCR during this period, to determine their prevalence in the HIV-positive patients.en_ZA
dc.identifier.apacitationDu Plessis, S. J. (2012). <i>Molecular characterisation of selected gastrointestinal microbiota in South African HIV-positive patients during HAART</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/10357en_ZA
dc.identifier.chicagocitationDu Plessis, Sarah Jane. <i>"Molecular characterisation of selected gastrointestinal microbiota in South African HIV-positive patients during HAART."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2012. http://hdl.handle.net/11427/10357en_ZA
dc.identifier.citationDu Plessis, S. 2012. Molecular characterisation of selected gastrointestinal microbiota in South African HIV-positive patients during HAART. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Du Plessis, Sarah Jane AB - Progression of the HIV disease is characterised by a massive depletion of CD4+ T cells and it has been shown that patients living with a more advanced HIV infection have a higher risk of developing diarrhoea due to the disruption of the gastrointestinal microbiota caused by either the HIV-infection or the use of antibiotics and drugs such as highly active antiretroviral therapy (HAART). An imbalance in the microbial composition, attributable to a disturbed mucosal barrier, as well as increased permeability and inflammation caused by HIV, can influence the metabolic (carbohydrate fermentation) and protective functions provided by the microbiota. The effect of HIV on the intestinal microbiota has not been widely examined and those studies that have focused on HIV and the gastrointestinal tract, have investigated it mainly from a virological perspective. Consequently, the aim of the study was to ascertain whether the diversity and/or abundance of the endogenous intestinal microbiota of South African HIV-positive patients was disrupted on account of HIV within the gastrointestinal tract. An additional aim was to determine whether the administration of HAART affected the microbiota during a 6 month longitudinal study. The diversity of the intestinal microbial composition was characterised with respect to the total bacteria, Bifidobacterium and Lactobacillus species using PCR-DGGE. qPCR was used to determine the abundance of total bacteria, Bifidobacterium, Lactobacillus, Escherichia coli, the Bacteroides/Prevotella, Clostridium coccoides and Clostridium leptum groups. ... In addition, three potential intestinal pathogens (Clostridium difficile, Campylobacter jejuni and Salmonella enterica) were monitored by qPCR during this period, to determine their prevalence in the HIV-positive patients. DA - 2012 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - Molecular characterisation of selected gastrointestinal microbiota in South African HIV-positive patients during HAART TI - Molecular characterisation of selected gastrointestinal microbiota in South African HIV-positive patients during HAART UR - http://hdl.handle.net/11427/10357 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/10357
dc.identifier.vancouvercitationDu Plessis SJ. Molecular characterisation of selected gastrointestinal microbiota in South African HIV-positive patients during HAART. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2012 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/10357en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleMolecular characterisation of selected gastrointestinal microbiota in South African HIV-positive patients during HAARTen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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