A study of biochemical and morphological aspects of macrophage function in experimental murine Nocardia asteroides and Nocardia brasiliensis infections

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dc.contributor.advisorFolb, Peter Ien_ZA
dc.contributor.authorStephens, Janeten_ZA
dc.date.accessioned2018-02-01T13:34:55Z
dc.date.available2018-02-01T13:34:55Z
dc.date.issued1987en_ZA
dc.description.abstractIt is submitted in this thesis that the degree of activation or inhibition of macrophage function may differ in N. asteroides and N. brasiliensis infections with respect to release of plasminogen activator and of lysozyme The pattern of secretion of plasminogen activator and lysozyme in N. asteroides infections appears to differ in N. brasiliensis infection; and there is possibly a difference in the amount of lysozyme released by 2 day N. asteroides-activated macrophages and 2 day N. brasiliensis -activated macrophages. Strains of Nocardia organism did not influence macrophage morphology or ultrastructure. The study also shows the biochemical characteristics of plasminogen activator and lysozyme release, but not macrophage morphology and ultrastructure, are modified in the first 21 days of experimental Nocardia infections. There are three apparent mechanisms by which virulent strains of N. asteroides manage to survive within macrophages: (i) an ability to inhibit phagosome-lysozome fusion: (ii) alteration in the intraphagosomal pH: and (iii) alteration in the activity of the lysozomal enzyme acid-phosphatase. This study attempted to elucidate further the mechanisms enabling Nocardia organisms to persist and grow within macrophages. Reduced lysozyme release reflects diminished functional status of the macrophages of mice inoculated with N. asteroides or N. brasiliensis at certain times during infection. Reduced intracellular lysozyme levels have been linked with defects in bactericidal function. Such a reduction in intracellular and consequently extracellular levels of lysozyme might explain the capacity of Nocardia to survive intracellularly and to proliferate in the macrophage host.en_ZA
dc.identifier.apacitationStephens, J. (1987). <i>A study of biochemical and morphological aspects of macrophage function in experimental murine Nocardia asteroides and Nocardia brasiliensis infections</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Clinical Pharmacology. Retrieved from http://hdl.handle.net/11427/27213en_ZA
dc.identifier.chicagocitationStephens, Janet. <i>"A study of biochemical and morphological aspects of macrophage function in experimental murine Nocardia asteroides and Nocardia brasiliensis infections."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Clinical Pharmacology, 1987. http://hdl.handle.net/11427/27213en_ZA
dc.identifier.citationStephens, J. 1987. A study of biochemical and morphological aspects of macrophage function in experimental murine Nocardia asteroides and Nocardia brasiliensis infections. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Stephens, Janet AB - It is submitted in this thesis that the degree of activation or inhibition of macrophage function may differ in N. asteroides and N. brasiliensis infections with respect to release of plasminogen activator and of lysozyme The pattern of secretion of plasminogen activator and lysozyme in N. asteroides infections appears to differ in N. brasiliensis infection; and there is possibly a difference in the amount of lysozyme released by 2 day N. asteroides-activated macrophages and 2 day N. brasiliensis -activated macrophages. Strains of Nocardia organism did not influence macrophage morphology or ultrastructure. The study also shows the biochemical characteristics of plasminogen activator and lysozyme release, but not macrophage morphology and ultrastructure, are modified in the first 21 days of experimental Nocardia infections. There are three apparent mechanisms by which virulent strains of N. asteroides manage to survive within macrophages: (i) an ability to inhibit phagosome-lysozome fusion: (ii) alteration in the intraphagosomal pH: and (iii) alteration in the activity of the lysozomal enzyme acid-phosphatase. This study attempted to elucidate further the mechanisms enabling Nocardia organisms to persist and grow within macrophages. Reduced lysozyme release reflects diminished functional status of the macrophages of mice inoculated with N. asteroides or N. brasiliensis at certain times during infection. Reduced intracellular lysozyme levels have been linked with defects in bactericidal function. Such a reduction in intracellular and consequently extracellular levels of lysozyme might explain the capacity of Nocardia to survive intracellularly and to proliferate in the macrophage host. DA - 1987 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1987 T1 - A study of biochemical and morphological aspects of macrophage function in experimental murine Nocardia asteroides and Nocardia brasiliensis infections TI - A study of biochemical and morphological aspects of macrophage function in experimental murine Nocardia asteroides and Nocardia brasiliensis infections UR - http://hdl.handle.net/11427/27213 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/27213
dc.identifier.vancouvercitationStephens J. A study of biochemical and morphological aspects of macrophage function in experimental murine Nocardia asteroides and Nocardia brasiliensis infections. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Clinical Pharmacology, 1987 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/27213en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Clinical Pharmacologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMacrophagesen_ZA
dc.subject.otherImmune responseen_ZA
dc.subject.otherBacterial diseasesen_ZA
dc.subject.otherNocardiaen_ZA
dc.subject.otherMice - Diseasesen_ZA
dc.subject.otherMacrophage activationen_ZA
dc.subject.otherMacrophagesen_ZA
dc.subject.otherMuridaeen_ZA
dc.subject.otherNocardia asteroidesen_ZA
dc.subject.otherNocardia infectionsen_ZA
dc.titleA study of biochemical and morphological aspects of macrophage function in experimental murine Nocardia asteroides and Nocardia brasiliensis infectionsen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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