RNA transmission and expression from inert HIV candidate vaccine virus-like-particles

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dc.contributor.advisorRybicki, Eden_ZA
dc.contributor.advisorMeyers, Annen_ZA
dc.contributor.advisorShephard, Eniden_ZA
dc.contributor.authorValley-Omar, Ziyaaden_ZA
dc.date.accessioned2014-07-30T17:42:14Z
dc.date.available2014-07-30T17:42:14Z
dc.date.issued2008en_ZA
dc.descriptionIncludes abstract.
dc.descriptionIncludes bibliographical references (leaves 136-158).
dc.description.abstractHIV-1 Gag virus-like-particles (VLPs) produced in various expression systems are potent stimulators of both cellular and humoral immune responses in animal models. The encapsidation of large concentrations of random cellular RNA species is known to accompany the assembly of HIV virus particles. This RNA plays a crucial role by serving as a molecular scaffold for the assembly of Gag structural proteins into particles. Non-pseudotyped VLPs that do not present any HIV envelope glycoproteins are regarded as inert particles as they contain no replicative nucleic acid and are presumed to be unable to deliver encapsidated RNA for expression in inoculated individuals. Live virus cellular entry studies have shown that non-pseudotyped Gag particles are destined for degradation in acidified vesicles subsequent to receptor independent cellular entry. In addition to host cell RNA incorporation, Gag VLPs produced in insect cell-based, baculovirus expression systems have been observed to incorporate the baculovirus-derived Gp64 envelope glycoprotein. Gp64 has been shown to be efficient at enabling the delivery and expression of genes from recombinant baculoviruses and other Gp64 pseudotyped live viruses in mammalian cell lines both in vivo and in vitro. This study, therefore, set out to establish for the first time whether inert, baculovirus-derived (Gp64 pseudotyped) Gag VLPs could mediate delivery and expression of randomly encapsidated RNAs in mammalian cell lines.en_ZA
dc.identifier.apacitationValley-Omar, Z. (2008). <i>RNA transmission and expression from inert HIV candidate vaccine virus-like-particles</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4345en_ZA
dc.identifier.chicagocitationValley-Omar, Ziyaad. <i>"RNA transmission and expression from inert HIV candidate vaccine virus-like-particles."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008. http://hdl.handle.net/11427/4345en_ZA
dc.identifier.citationValley-Omar, Z. 2008. RNA transmission and expression from inert HIV candidate vaccine virus-like-particles. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Valley-Omar, Ziyaad AB - HIV-1 Gag virus-like-particles (VLPs) produced in various expression systems are potent stimulators of both cellular and humoral immune responses in animal models. The encapsidation of large concentrations of random cellular RNA species is known to accompany the assembly of HIV virus particles. This RNA plays a crucial role by serving as a molecular scaffold for the assembly of Gag structural proteins into particles. Non-pseudotyped VLPs that do not present any HIV envelope glycoproteins are regarded as inert particles as they contain no replicative nucleic acid and are presumed to be unable to deliver encapsidated RNA for expression in inoculated individuals. Live virus cellular entry studies have shown that non-pseudotyped Gag particles are destined for degradation in acidified vesicles subsequent to receptor independent cellular entry. In addition to host cell RNA incorporation, Gag VLPs produced in insect cell-based, baculovirus expression systems have been observed to incorporate the baculovirus-derived Gp64 envelope glycoprotein. Gp64 has been shown to be efficient at enabling the delivery and expression of genes from recombinant baculoviruses and other Gp64 pseudotyped live viruses in mammalian cell lines both in vivo and in vitro. This study, therefore, set out to establish for the first time whether inert, baculovirus-derived (Gp64 pseudotyped) Gag VLPs could mediate delivery and expression of randomly encapsidated RNAs in mammalian cell lines. DA - 2008 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - RNA transmission and expression from inert HIV candidate vaccine virus-like-particles TI - RNA transmission and expression from inert HIV candidate vaccine virus-like-particles UR - http://hdl.handle.net/11427/4345 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4345
dc.identifier.vancouvercitationValley-Omar Z. RNA transmission and expression from inert HIV candidate vaccine virus-like-particles. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4345en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleRNA transmission and expression from inert HIV candidate vaccine virus-like-particlesen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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