A proteomic investigation of the immune response of the South African abalone, Haliotis midae

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dc.contributor.advisorCoyne, Vernonen_ZA
dc.contributor.authorBeltran, Caroline Gina Gracieuseen_ZA
dc.date.accessioned2016-01-21T11:12:39Z
dc.date.available2016-01-21T11:12:39Z
dc.date.issued2015en_ZA
dc.descriptionIncludes bibliographical referencesen_ZA
dc.description.abstractHaliotis midae is a commercially important abalone in South Africa, previously harvested from a stable, quota-managed fishery. However, the combined effects of overharvesting, increased illegal catches and negative environmental factors led to a collapse in wild populations in the mid-90s. Consequently, land-based aquaculture of H. midae has grown significantly in South Africa, to satisfy the global demand for abalone and alleviate pressure on wild stocks. Unfortunately, disease outbreaks have had a severe impact on the abalone aquaculture industry internationally and remain one of the single biggest factors contributing to economic loss. Understanding the effects of pathogen infection of abalone is therefore crucial to mitigating and controlling infection outbreaks on farms. Despite this, the molecular mechanisms underlying the immune response of H. midae remain obscure. High-throughput proteomics, a powerful tool to analyse global protein expression changes, can provide an integrated view of the immune system. Thus, this study aimed to elucidate the haemocyte proteome of H. midae and gain insight into regulatory molecular pathways underlying innate immunity. In this study, a comparative shotgun proteomics approach using isobaric tagging for relative and absolute quantification (iTRAQ) coupled with LC-MS/MS was employed to investigate H. midae proteome changes in response to Vibrio anguillarum challenge. A preliminary iTRAQ challenge trial was conducted which identified a putative early (1 and 2 hours post-injection) and late (48 hours post-injection) proteome response to bacterial-challenge. Using these time points, four independent challenge trials were conducted and analysed by iTRAQ and the results combined to produce a high-confidence dataset with good quantitative reproducibility for statistical analysis. A parallel set of experiments was conducted using mock-infected samples.en_ZA
dc.identifier.apacitationBeltran, C. G. G. (2015). <i>A proteomic investigation of the immune response of the South African abalone, Haliotis midae</i>. (Thesis). University of Cape Town ,Faculty of Humanities ,Department of English Language and Literature. Retrieved from http://hdl.handle.net/11427/16483en_ZA
dc.identifier.chicagocitationBeltran, Caroline Gina Gracieuse. <i>"A proteomic investigation of the immune response of the South African abalone, Haliotis midae."</i> Thesis., University of Cape Town ,Faculty of Humanities ,Department of English Language and Literature, 2015. http://hdl.handle.net/11427/16483en_ZA
dc.identifier.citationBeltran, C. 2015. A proteomic investigation of the immune response of the South African abalone, Haliotis midae. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Beltran, Caroline Gina Gracieuse AB - Haliotis midae is a commercially important abalone in South Africa, previously harvested from a stable, quota-managed fishery. However, the combined effects of overharvesting, increased illegal catches and negative environmental factors led to a collapse in wild populations in the mid-90s. Consequently, land-based aquaculture of H. midae has grown significantly in South Africa, to satisfy the global demand for abalone and alleviate pressure on wild stocks. Unfortunately, disease outbreaks have had a severe impact on the abalone aquaculture industry internationally and remain one of the single biggest factors contributing to economic loss. Understanding the effects of pathogen infection of abalone is therefore crucial to mitigating and controlling infection outbreaks on farms. Despite this, the molecular mechanisms underlying the immune response of H. midae remain obscure. High-throughput proteomics, a powerful tool to analyse global protein expression changes, can provide an integrated view of the immune system. Thus, this study aimed to elucidate the haemocyte proteome of H. midae and gain insight into regulatory molecular pathways underlying innate immunity. In this study, a comparative shotgun proteomics approach using isobaric tagging for relative and absolute quantification (iTRAQ) coupled with LC-MS/MS was employed to investigate H. midae proteome changes in response to Vibrio anguillarum challenge. A preliminary iTRAQ challenge trial was conducted which identified a putative early (1 and 2 hours post-injection) and late (48 hours post-injection) proteome response to bacterial-challenge. Using these time points, four independent challenge trials were conducted and analysed by iTRAQ and the results combined to produce a high-confidence dataset with good quantitative reproducibility for statistical analysis. A parallel set of experiments was conducted using mock-infected samples. DA - 2015 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - A proteomic investigation of the immune response of the South African abalone, Haliotis midae TI - A proteomic investigation of the immune response of the South African abalone, Haliotis midae UR - http://hdl.handle.net/11427/16483 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16483
dc.identifier.vancouvercitationBeltran CGG. A proteomic investigation of the immune response of the South African abalone, Haliotis midae. [Thesis]. University of Cape Town ,Faculty of Humanities ,Department of English Language and Literature, 2015 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/16483en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of English Language and Literatureen_ZA
dc.publisher.facultyFaculty of Humanitiesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleA proteomic investigation of the immune response of the South African abalone, Haliotis midaeen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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