Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis

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dc.contributor.advisorFarrant, Jill Men_ZA
dc.contributor.advisorIlling, Nicolaen_ZA
dc.contributor.authorGinbot, Zekarias Gebremedhinen_ZA
dc.date.accessioned2014-12-30T06:42:31Z
dc.date.available2014-12-30T06:42:31Z
dc.date.issued2011en_ZA
dc.description.abstractStudies on resurrection plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and function of XhLEA1-4S1 and XhLEA1-1S2, Group 1 LEA proteins from Xerophyta humilis, in order to shed light on their role in desiccation tolerance. Complementary DNA (cDNA) of these XhLEAs were cloned into bacterial expression vectors and the recombinant proteins expressed in E. coli. Antibodies were generated and used in determination of expression conditions and immunolocalization studies.en_ZA
dc.identifier.apacitationGinbot, Z. G. (2011). <i>Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/10523en_ZA
dc.identifier.chicagocitationGinbot, Zekarias Gebremedhin. <i>"Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011. http://hdl.handle.net/11427/10523en_ZA
dc.identifier.citationGinbot, Z. 2011. Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Ginbot, Zekarias Gebremedhin AB - Studies on resurrection plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and function of XhLEA1-4S1 and XhLEA1-1S2, Group 1 LEA proteins from Xerophyta humilis, in order to shed light on their role in desiccation tolerance. Complementary DNA (cDNA) of these XhLEAs were cloned into bacterial expression vectors and the recombinant proteins expressed in E. coli. Antibodies were generated and used in determination of expression conditions and immunolocalization studies. DA - 2011 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis TI - Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis UR - http://hdl.handle.net/11427/10523 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/10523
dc.identifier.vancouvercitationGinbot ZG. Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/10523en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleCharacterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilisen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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