Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli

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dc.contributor.advisorWoods, David Ren_ZA
dc.contributor.authorScholle, Renate Reginaen_ZA
dc.date.accessioned2016-09-25T16:47:13Z
dc.date.available2016-09-25T16:47:13Z
dc.date.issued1989en_ZA
dc.descriptionBibliography: pages 143-154.en_ZA
dc.description.abstractThis dissertation forms part of the study on the molecular biology of the aerobic, collagenolytic, halotolerant, gram-negative organism Vibrio alginolyticus. The cloning, expression and regulation of the v. alginolyticus sucrose utilization system in Escherichia coli is discussed and the results of a molecular analysis of the sucrase gene (scrA) are presented. The clone pVSlOO, containing a sucrose utilization system, was isolated from a genomic library of v. alginolyticus. Plasmid pVSlOO was mapped and the origin of its insert determined by Southern blotting and DNA hybridization. The number and sizes of the polypeptide products encoded by plasmid pVSlOO were determined by DNAdirected cell-free protein synthesis. The capsule, produced by transformed and untransformed E. coli JA221 cells, was shown to be independent of the presence of plasmid pVSlOO. The sucrase activity assay was optimized with respect to time, pH, temperature and salt requirements.en_ZA
dc.identifier.apacitationScholle, R. R. (1989). <i>Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/21906en_ZA
dc.identifier.chicagocitationScholle, Renate Regina. <i>"Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1989. http://hdl.handle.net/11427/21906en_ZA
dc.identifier.citationScholle, R. 1989. Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Scholle, Renate Regina AB - This dissertation forms part of the study on the molecular biology of the aerobic, collagenolytic, halotolerant, gram-negative organism Vibrio alginolyticus. The cloning, expression and regulation of the v. alginolyticus sucrose utilization system in Escherichia coli is discussed and the results of a molecular analysis of the sucrase gene (scrA) are presented. The clone pVSlOO, containing a sucrose utilization system, was isolated from a genomic library of v. alginolyticus. Plasmid pVSlOO was mapped and the origin of its insert determined by Southern blotting and DNA hybridization. The number and sizes of the polypeptide products encoded by plasmid pVSlOO were determined by DNAdirected cell-free protein synthesis. The capsule, produced by transformed and untransformed E. coli JA221 cells, was shown to be independent of the presence of plasmid pVSlOO. The sucrase activity assay was optimized with respect to time, pH, temperature and salt requirements. DA - 1989 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1989 T1 - Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli TI - Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli UR - http://hdl.handle.net/11427/21906 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21906
dc.identifier.vancouvercitationScholle RR. Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1989 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21906en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherVibrioen_ZA
dc.subject.otherEscherichia colien_ZA
dc.subject.otherSucroseen_ZA
dc.titleStudies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia colien_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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