The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation
Doctoral Thesis
2002
Permanent link to this Item
Authors
Supervisors
Journal Title
Link to Journal
Journal ISSN
Volume Title
Publisher
Publisher
University of Cape Town
Department
Faculty
License
Series
Abstract
The transfer of T-DNA from Agrobacterium tumefaciens to eukaryotic cells is on of the most extensively studied examples of interkingdom DNA transfer. This transfer system has revolutionized plant molecular biology to the extent that the introduction of foreign genes into plant genomes is now a basic technique. Apart from the finding that a defined segment (T-DNA) of the A. tumefaciens Ti plasmid can be transferred to plant DNA, the discovery that the mobilization functions of the IncQ plasmid, RSF1010, can mediate its transfer from A. tumefaciens into plant cells as well between Gram negative bacteria has generated considerable interest. The mobilization functions of RSF1010 substitute for the requirement of the 25-bp border T-DNA sequences of A. tumefaciens. In this thesis I investigated the ability of another plasmid, pTF-FC2, to transfer genes to plants.
Description
Bibliography: leaves 165-195.
Reference:
Dube, T. 2002. The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation. University of Cape Town.