Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats

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dc.contributor.advisorPimstone, Bernard Len_ZA
dc.contributor.authorShapiro, Brahmen_ZA
dc.date.accessioned2018-03-15T07:38:00Z
dc.date.available2018-03-15T07:38:00Z
dc.date.issued1978en_ZA
dc.description.abstractThe studies presented in this thesis represent an attempt to characterise some aspects of the abnormalities of somatomedin physiology in protein-energy malnutrition. A suitable bioassay to study somatomedin was found in that of Van den Brande and Du Caju (1974) which makes use of uniform discs of cartilage punched from slices of immature porcine costal cartilage. The handling of the cartilage discs was made easy by the specially designed incubation rack. Preincubation increased sensitivity of the cartilage, and post incubation with radioactive tracer reduced the potential interference of varying sulphate content of assay samples. Serum samples were subjected to formal multipoint parallel line bioassay and data analysed by a computer programme designed to examine such assays. The bioassay was found to be sensitive to a concentration of serum of 5% and to have an index precision of less than 0.30 (and usually less than 0.20).en_ZA
dc.identifier.apacitationShapiro, B. (1978). <i>Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Endocrinology and Diabetology. Retrieved from http://hdl.handle.net/11427/27657en_ZA
dc.identifier.chicagocitationShapiro, Brahm. <i>"Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Endocrinology and Diabetology, 1978. http://hdl.handle.net/11427/27657en_ZA
dc.identifier.citationShapiro, B. 1978. Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Shapiro, Brahm AB - The studies presented in this thesis represent an attempt to characterise some aspects of the abnormalities of somatomedin physiology in protein-energy malnutrition. A suitable bioassay to study somatomedin was found in that of Van den Brande and Du Caju (1974) which makes use of uniform discs of cartilage punched from slices of immature porcine costal cartilage. The handling of the cartilage discs was made easy by the specially designed incubation rack. Preincubation increased sensitivity of the cartilage, and post incubation with radioactive tracer reduced the potential interference of varying sulphate content of assay samples. Serum samples were subjected to formal multipoint parallel line bioassay and data analysed by a computer programme designed to examine such assays. The bioassay was found to be sensitive to a concentration of serum of 5% and to have an index precision of less than 0.30 (and usually less than 0.20). DA - 1978 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1978 T1 - Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats TI - Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats UR - http://hdl.handle.net/11427/27657 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/27657
dc.identifier.vancouvercitationShapiro B. Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Endocrinology and Diabetology, 1978 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/27657en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Endocrinology and Diabetologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherSomatomedinsen_ZA
dc.subject.otherProtein deficiencyen_ZA
dc.titleSerum somatomedin and somatomedin generation by the perfused liver in protein malnourished ratsen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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