Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species

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dc.contributor.advisorSewell, Trevoren_ZA
dc.contributor.authorKimani, Serahen_ZA
dc.date.accessioned2015-01-01T12:38:51Z
dc.date.available2015-01-01T12:38:51Z
dc.date.issued2011en_ZA
dc.description.abstractTo investigate the structural determinants of NitN specificity on short aliphatic amide substrates by analyzing binding and interactions of these molecules with the NitN binding pocket. To probe the catalytic role of the two active site glutamate residues (Glu61 and Glu139) using NitN as a model enzyme. To monitor the activity, interactions and reactivity of the WT NitN and the Glu61 and Glu139 NitN mutants with ACR.en_ZA
dc.identifier.apacitationKimani, S. (2011). <i>Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/10837en_ZA
dc.identifier.chicagocitationKimani, Serah. <i>"Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011. http://hdl.handle.net/11427/10837en_ZA
dc.identifier.citationKimani, S. 2011. Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Kimani, Serah AB - To investigate the structural determinants of NitN specificity on short aliphatic amide substrates by analyzing binding and interactions of these molecules with the NitN binding pocket. To probe the catalytic role of the two active site glutamate residues (Glu61 and Glu139) using NitN as a model enzyme. To monitor the activity, interactions and reactivity of the WT NitN and the Glu61 and Glu139 NitN mutants with ACR. DA - 2011 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species TI - Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species UR - http://hdl.handle.net/11427/10837 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/10837
dc.identifier.vancouvercitationKimani S. Catalysis, substrate binding and specificity in the amidase from Nesterenkonia species. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/10837en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleCatalysis, substrate binding and specificity in the amidase from Nesterenkonia speciesen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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