Antibacterial and antimycobacterial activities of South African Salvia species and isolated compounds from S. chamelaeagnea

Seaman, T; Kamatou, G P P; Van Vuuren, S F; Van Heerden, F R; Viljoen, A M

Antibacterial and antimycobacterial activities of South African Salvia species and isolated compounds from S. chamelaeagnea

Journal Article

2007

Journal Title

South African Journal of Botany

Link to Journal

http://www.sciencedirect.com/science/article/pii/S0254629907003079

Publisher

University of Cape Town

Department

Division of Clinical Pharmacology

Faculty

Faculty of Health Sciences

Abstract

Extracts of 16 South African Salvia species commonly used in traditional medicine to treat various microbial infections were investigated for in vitro antibacterial and antimycobacterial activities using the micro-dilution and respiratory BACTEC method, respectively. The micro-organisms tested include two Gram-positive (Staphylococcus aureus and Bacillus cereus); two Gram-negative (Escherichia coli and Klebsiella pneumoniae) bacterial strains and the common pathogen responsible for tuberculosis, Mycobacterium tuberculosis. Extracts of the majority of species exhibited moderate to good antibacterial activity with minimum inhibitory concentration (MIC) values ranging from 0.03 to 8.00 mg/ml. Promising activity was observed against M. tuberculosis (MIC ≤ 0.50 mg/ml) with S. radula, S. verbenaca and S. dolomitica displaying the most favourable activity (MIC: 0.10 mg/ml). The antibacterial bioassay-guided fractionation of S. chamelaeagnea resulted in the isolation of four compounds: carnosol, 7-O-methylepirosmanol, oleanolic acid and its isomer ursolic acid as the active principles against S. aureus. The in vitro antibacterial and antimycobacterial activities may support the use of Salvia species in traditional medicine to treat microbial infections.