The reconstitution of the histone octamer

dc.contributor.advisorVon Holt, Clausen_ZA
dc.contributor.advisorSewell, Bryan Trevoren_ZA
dc.contributor.authorGreyling, H Jen_ZA
dc.date.accessioned2016-11-16T13:22:57Z
dc.date.available2016-11-16T13:22:57Z
dc.date.issued1987en_ZA
dc.descriptionBibliography: pages 110-126.en_ZA
dc.description.abstractThis thesis describes methodology for the reconstitution of the chicken erythrocyte octamer from acid-denatured histones or the natural H3-H4 tetramer and H2A-H2B dimers. Oligomeric properties of reconstituted octamers were elucidated during column chromatographic and chemical cross-linking studies. The conformational identity of the natural and reconstituted octamers was demonstrated by the ability of all preparations to crystallise as helical octamer tubes. The application of the reconstitution methodology in addressing fundamental problems of chromatin research, was demonstrated during subsequent studies, namely (i) The reconstitution of hybrid histone octamers containing a structural variant of a specific histone. These studies were undertaken to study the effect on histone-histone interactions in hybrid octamers of which erythrocyte H2B was substituted for by sea urchin sperm H2B(l) or erythrocyte H3 and H4 were substituted for by dethiolated H3 and sea urchin sperm H4 respectively. (ii) The reconstitution of an octamer suitable for the sitespecific derivatisation of a specific histone, or covalently labelled with aurothiomalate in a specific histone complex. These studies were concluded to represent general labelling strategies which may be of use in crystallographic or physico-chemical studies of nucleosome structure.en_ZA
dc.identifier.apacitationGreyling, H. J. (1987). <i>The reconstitution of the histone octamer</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/22553en_ZA
dc.identifier.chicagocitationGreyling, H J. <i>"The reconstitution of the histone octamer."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1987. http://hdl.handle.net/11427/22553en_ZA
dc.identifier.citationGreyling, H. 1987. The reconstitution of the histone octamer. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Greyling, H J AB - This thesis describes methodology for the reconstitution of the chicken erythrocyte octamer from acid-denatured histones or the natural H3-H4 tetramer and H2A-H2B dimers. Oligomeric properties of reconstituted octamers were elucidated during column chromatographic and chemical cross-linking studies. The conformational identity of the natural and reconstituted octamers was demonstrated by the ability of all preparations to crystallise as helical octamer tubes. The application of the reconstitution methodology in addressing fundamental problems of chromatin research, was demonstrated during subsequent studies, namely (i) The reconstitution of hybrid histone octamers containing a structural variant of a specific histone. These studies were undertaken to study the effect on histone-histone interactions in hybrid octamers of which erythrocyte H2B was substituted for by sea urchin sperm H2B(l) or erythrocyte H3 and H4 were substituted for by dethiolated H3 and sea urchin sperm H4 respectively. (ii) The reconstitution of an octamer suitable for the sitespecific derivatisation of a specific histone, or covalently labelled with aurothiomalate in a specific histone complex. These studies were concluded to represent general labelling strategies which may be of use in crystallographic or physico-chemical studies of nucleosome structure. DA - 1987 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1987 T1 - The reconstitution of the histone octamer TI - The reconstitution of the histone octamer UR - http://hdl.handle.net/11427/22553 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/22553
dc.identifier.vancouvercitationGreyling HJ. The reconstitution of the histone octamer. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1987 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/22553en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherHistonesen_ZA
dc.subject.otherErythrocytes - Histochemistryen_ZA
dc.titleThe reconstitution of the histone octameren_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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