Construction and characterization of chimaeric human immunodefiency virus type 1 subtype C Gag virus-like particles

Master Thesis

2006

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http://hdl.handle.net/11427/4269
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thesis_sci_2006_halsey_rj.pdf (24.96 MB)
Authors
Halsey, Richard James
Supervisors
Tanzer, Fiona
Jaffray, Ann
Rybicki, Ed
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University of Cape Town

Department
Department of Molecular and Cell Biology
Faculty
Faculty of Science
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Abstract
In this study I explored the possibility of making HIV-1 subtype C Pr55gag-based chimaeric virus-like particles (VLPs) as a boost to the HIV-IC multigene DNA vaccine pTHr.grttnC, which encodes a modified Gag-RT-Tat-Nef fusion protein (GRTTN). Furthermore, an attempt was made to produce VLP analogues to the HIV-IA polyepitope DNA vaccine pTHr.HIVA. A range of in-frame fusions with the C-termini of myristylation-competent p6-truncated Gag and native Pr55gag were made to test how the length of polypeptide and its sequence might affect VLP formation and structure.
Description

Includes bibliographical references (leaves 114-128).

Keywords
Cell Biology

Reference:

Halsey, R. 2006. Construction and characterization of chimaeric human immunodefiency virus type 1 subtype C Gag virus-like particles. University of Cape Town.

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