Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection

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dc.contributor.advisorBrombacher, Franken_ZA
dc.contributor.advisorMagez, Sefanen_ZA
dc.contributor.authorBarkhuizen, Marken_ZA
dc.date.accessioned2014-07-28T14:54:13Z
dc.date.available2014-07-28T14:54:13Z
dc.date.issued2008en_ZA
dc.descriptionIncludes bibliographical references.
dc.description.abstractAfrican trypanosomiasis encompasses diseases caused by pathogenic trypanosomes, infecting both humans and animals alike. To determine the immunological role of IL=12 family members in Trypanosoma brucei brucei, Trypanosoma evansi and Trypanosoma congolense infections, IL-12p35¯/¯, IL-12p40¯/¯ and IL-12p35¯/¯/p40¯/¯ mice were used. While the two latter mouse strains lack all IL-12 homologues, IL-12p35¯/¯ mice still produce IL-12p80 homodimers and IL-23. In infection with T.b. brucei and T.evansi; IL-12p35¯/¯, IL-12p40¯/¯ or IL-12p35¯/¯/p40¯/¯ mice were susceptible to both these pathogens, demonstrated by increased mortality compared to wild type C57BL/6 mice. The different IL-12 deficient mouse strains showed similar mortality kinetics, suggesting that IL-12p70 but not the IL-12p80 homodimer or IL-23 plays a crucial role in survival. Similarly, parasitemia control was reduced in the absence of IL-12p70. While plasma levels of IgM and IgG2c were similar between IL-12 deficient mice and wild type mice, IF-γ production. As IFN-γR¯/¯ mice were also highly susceptible to both T.b. brucei and T. evansi, IL-12p70-dependent IFN-γ production seems to be important mechanism involved in resistance against both these pathogens.en_ZA
dc.identifier.apacitationBarkhuizen, M. (2008). <i>Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Immunology. Retrieved from http://hdl.handle.net/11427/3119en_ZA
dc.identifier.chicagocitationBarkhuizen, Mark. <i>"Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Immunology, 2008. http://hdl.handle.net/11427/3119en_ZA
dc.identifier.citationBarkhuizen, M. 2008. Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Barkhuizen, Mark AB - African trypanosomiasis encompasses diseases caused by pathogenic trypanosomes, infecting both humans and animals alike. To determine the immunological role of IL=12 family members in Trypanosoma brucei brucei, Trypanosoma evansi and Trypanosoma congolense infections, IL-12p35¯/¯, IL-12p40¯/¯ and IL-12p35¯/¯/p40¯/¯ mice were used. While the two latter mouse strains lack all IL-12 homologues, IL-12p35¯/¯ mice still produce IL-12p80 homodimers and IL-23. In infection with T.b. brucei and T.evansi; IL-12p35¯/¯, IL-12p40¯/¯ or IL-12p35¯/¯/p40¯/¯ mice were susceptible to both these pathogens, demonstrated by increased mortality compared to wild type C57BL/6 mice. The different IL-12 deficient mouse strains showed similar mortality kinetics, suggesting that IL-12p70 but not the IL-12p80 homodimer or IL-23 plays a crucial role in survival. Similarly, parasitemia control was reduced in the absence of IL-12p70. While plasma levels of IgM and IgG2c were similar between IL-12 deficient mice and wild type mice, IF-γ production. As IFN-γR¯/¯ mice were also highly susceptible to both T.b. brucei and T. evansi, IL-12p70-dependent IFN-γ production seems to be important mechanism involved in resistance against both these pathogens. DA - 2008 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection TI - Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection UR - http://hdl.handle.net/11427/3119 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/3119
dc.identifier.vancouvercitationBarkhuizen M. Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Immunology, 2008 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/3119en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Immunologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherImmunologyen_ZA
dc.titleDetermination of the role of cytokines using gene deficient mice in African trypanosomiasis infectionen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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