Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity

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dc.contributor.advisorMeyers, Paulen_ZA
dc.contributor.advisorGammon, David Wen_ZA
dc.contributor.advisorFranca, Marie-Geneviève Dijouxen_ZA
dc.contributor.authorBamuamba, Kapinga Benoiten_ZA
dc.date.accessioned2014-07-30T17:35:33Z
dc.date.available2014-07-30T17:35:33Z
dc.date.issued2006en_ZA
dc.descriptionIncludes bibliographical references (leaves 126-140).
dc.description.abstractIn our search for new anti-tuberculosis lead molecules, five medicinal plant species, Olea capensis (L.l, Tulbaghia alliacea (L.), Inula graveolens (L.), Leyssera gnaphaloides (L.), and Buddleja saligna (L.) were collected in Cape Town and surrounding area and investigated for antimycobacterial activity following report of their therapeutic use in traditional medicine to treat infectious diseases such as tuberculosis. A bioassay guided fractionation of the acetone/water (4:1) crude extracts of O. capensis (leaves) and T. alliacea (rhizomes) showed no activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 252923, and Mycobacterium aurum A+. In contrast, the orgamc fractions (hexane, dichloromethane) of the acetone/water (4: 1) crude extracts of 1. graveolens, L. gnaphaloides, and B. saligna exhibited significant activity against M. tuberculosis H37Rv, M. avium 25291, M. microti ATCC 19422, and M. scrofulaceum ATCC 19987. The isolation and structure determination of the bioactive led to the identification of pentacyclic triterpenoids, ursolic acid (UA) and oleanolic acid as major antitubercular constituents of B. saligna, L. gnaphaloides, and 1. graveolens. The in vitro cytotoxicity assays of the isolated bioactive constituents showed no cytotoxicity against Chinese Hamster Ovarian (CHO) cells line. Subsequently, given the pharmaceutical value of the above finding, a survey on structure activity of pentacyclic triterpenoids was conducted. It was was found, for instance that selective substitutions at C-3 and/or C-28 and the double bond at UA, OA and betulinic and (1) BA) were made in order to improve anti-tumour and anti-HIV activity. However, thought a great number of modified bioactive pentacyclic triterpenoids is reported, none was tested against Mtb. Therefore, this study also explored a new synthetic route (scheme 1) toward a generation of (5), which may allow improving antitubercular, anti-HIV or anti-tumour activity, and/or specificity.en_ZA
dc.identifier.apacitationBamuamba, K. B. (2006). <i>Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4241en_ZA
dc.identifier.chicagocitationBamuamba, Kapinga Benoit. <i>"Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2006. http://hdl.handle.net/11427/4241en_ZA
dc.identifier.citationBamuamba, K. 2006. Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Bamuamba, Kapinga Benoit AB - In our search for new anti-tuberculosis lead molecules, five medicinal plant species, Olea capensis (L.l, Tulbaghia alliacea (L.), Inula graveolens (L.), Leyssera gnaphaloides (L.), and Buddleja saligna (L.) were collected in Cape Town and surrounding area and investigated for antimycobacterial activity following report of their therapeutic use in traditional medicine to treat infectious diseases such as tuberculosis. A bioassay guided fractionation of the acetone/water (4:1) crude extracts of O. capensis (leaves) and T. alliacea (rhizomes) showed no activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 252923, and Mycobacterium aurum A+. In contrast, the orgamc fractions (hexane, dichloromethane) of the acetone/water (4: 1) crude extracts of 1. graveolens, L. gnaphaloides, and B. saligna exhibited significant activity against M. tuberculosis H37Rv, M. avium 25291, M. microti ATCC 19422, and M. scrofulaceum ATCC 19987. The isolation and structure determination of the bioactive led to the identification of pentacyclic triterpenoids, ursolic acid (UA) and oleanolic acid as major antitubercular constituents of B. saligna, L. gnaphaloides, and 1. graveolens. The in vitro cytotoxicity assays of the isolated bioactive constituents showed no cytotoxicity against Chinese Hamster Ovarian (CHO) cells line. Subsequently, given the pharmaceutical value of the above finding, a survey on structure activity of pentacyclic triterpenoids was conducted. It was was found, for instance that selective substitutions at C-3 and/or C-28 and the double bond at UA, OA and betulinic and (1) BA) were made in order to improve anti-tumour and anti-HIV activity. However, thought a great number of modified bioactive pentacyclic triterpenoids is reported, none was tested against Mtb. Therefore, this study also explored a new synthetic route (scheme 1) toward a generation of (5), which may allow improving antitubercular, anti-HIV or anti-tumour activity, and/or specificity. DA - 2006 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2006 T1 - Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity TI - Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity UR - http://hdl.handle.net/11427/4241 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4241
dc.identifier.vancouvercitationBamuamba KB. Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2006 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4241en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titlePharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activityen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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