The amino acid sequence of chicken histone F3

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dc.contributor.advisorVon Holt, Clausen_ZA
dc.contributor.authorBrandt, Wolf Friedrichen_ZA
dc.date.accessioned2016-03-14T07:14:41Z
dc.date.available2016-03-14T07:14:41Z
dc.date.issued1973en_ZA
dc.description.abstractHistone F3 (III) from chicken erythrocytes was isolated by selective extraction from nucleoprotein with ethanolic-HCl and purified by a single gel filtration step. This protein was found to be homogeneous by the following criteria: gel filtration, electrophoretic mobility, N- and C-terminal amino acid residues and amino acid analysis. The primary structure of this histone was established without resorting to the use of overlapping sequences. This has been achieved with specific chemical cleavages rather than enzymatic degradations chosen and applied, first to the original protein chain, and subsequently to the generated polypeptides, to yield sets of not more than 3 peptides in any single cleavage. Their relative position in the protein or polypeptides became evident after comparison of the N- and C-terminal amino acids in the cleavage products and the uncleaved starting material. The simplicity of the peptide mixture after each cleavage, resulting in easy separation of the peptides, together with the highly efficient Edman degradation of automatic sequencing, allowed a rapid and relatively nonlaborious primary structure determination. Finally, the amino acid sequence is compared with those of protamines and other histones. The evolution and the structure of this protein in relation to DNA is briefly considered.en_ZA
dc.identifier.apacitationBrandt, W. F. (1973). <i>The amino acid sequence of chicken histone F3</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/17699en_ZA
dc.identifier.chicagocitationBrandt, Wolf Friedrich. <i>"The amino acid sequence of chicken histone F3."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1973. http://hdl.handle.net/11427/17699en_ZA
dc.identifier.citationBrandt, W. 1973. The amino acid sequence of chicken histone F3. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Brandt, Wolf Friedrich AB - Histone F3 (III) from chicken erythrocytes was isolated by selective extraction from nucleoprotein with ethanolic-HCl and purified by a single gel filtration step. This protein was found to be homogeneous by the following criteria: gel filtration, electrophoretic mobility, N- and C-terminal amino acid residues and amino acid analysis. The primary structure of this histone was established without resorting to the use of overlapping sequences. This has been achieved with specific chemical cleavages rather than enzymatic degradations chosen and applied, first to the original protein chain, and subsequently to the generated polypeptides, to yield sets of not more than 3 peptides in any single cleavage. Their relative position in the protein or polypeptides became evident after comparison of the N- and C-terminal amino acids in the cleavage products and the uncleaved starting material. The simplicity of the peptide mixture after each cleavage, resulting in easy separation of the peptides, together with the highly efficient Edman degradation of automatic sequencing, allowed a rapid and relatively nonlaborious primary structure determination. Finally, the amino acid sequence is compared with those of protamines and other histones. The evolution and the structure of this protein in relation to DNA is briefly considered. DA - 1973 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1973 T1 - The amino acid sequence of chicken histone F3 TI - The amino acid sequence of chicken histone F3 UR - http://hdl.handle.net/11427/17699 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/17699
dc.identifier.vancouvercitationBrandt WF. The amino acid sequence of chicken histone F3. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1973 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/17699en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleThe amino acid sequence of chicken histone F3en_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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