Deoxyribonuclease probing on sea urchin embryo chromatin

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dc.contributor.authorLandsman, Daviden_ZA
dc.date.accessioned2016-03-09T09:05:51Z
dc.date.available2016-03-09T09:05:51Z
dc.date.issued1983en_ZA
dc.descriptionBibliography: pages 118-143.en_ZA
dc.description.abstractThe role that the sea urchin, Parechinus angulosus, embryo and sperm histone variants play in chromatin structure has been investigated. Chromatin structure has been determined at different levels of resolution in sperm and in developing embryos using micrococcal nuclease, pancreatic deoxyribonuclease (DNase I) and restriction endonucleases. Micrococcal nuclease and restriction endonuclease digestions of sea urchin gastrula chromatin have been analysed and it is shown that it is not possible to isolate large polynucleosomal chromatin complexes which are soluble in low ionic strength buffers. The nucleosomal DNA repeat lengths for sea urchin blastula, gastrula and sperm have been determined using micrococcal nuclease. The repeat length for sperm is significantly larger than blastula and gastrula repeat lengths whereas blastula and gastrula repeat lengths are not significantly different. Nucleosomal core particles have been isolated from early blastula, gastrula and sperm of sea urchins. After DNase I digestion of 51-labelled core particles the rate constants of cutting of the DNA at the susceptible sites on these core particles have been determined. The DNase I digestion kinetics of blastula and gastrula core particles are similar whereas sperm core particles are digested at a slower rate, mainly at the sites which are closest to the ends of the core particle DNA. Also, a site, which is 5 bases on the outside of the core particle and which is partially protected from nuclease attack, has been identified. The implications of these findings in relation to the histone variants in embryos and sperm of sea urchins are discussed.en_ZA
dc.identifier.apacitationLandsman, D. (1983). <i>Deoxyribonuclease probing on sea urchin embryo chromatin</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/17600en_ZA
dc.identifier.chicagocitationLandsman, David. <i>"Deoxyribonuclease probing on sea urchin embryo chromatin."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1983. http://hdl.handle.net/11427/17600en_ZA
dc.identifier.citationLandsman, D. 1983. Deoxyribonuclease probing on sea urchin embryo chromatin. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Landsman, David AB - The role that the sea urchin, Parechinus angulosus, embryo and sperm histone variants play in chromatin structure has been investigated. Chromatin structure has been determined at different levels of resolution in sperm and in developing embryos using micrococcal nuclease, pancreatic deoxyribonuclease (DNase I) and restriction endonucleases. Micrococcal nuclease and restriction endonuclease digestions of sea urchin gastrula chromatin have been analysed and it is shown that it is not possible to isolate large polynucleosomal chromatin complexes which are soluble in low ionic strength buffers. The nucleosomal DNA repeat lengths for sea urchin blastula, gastrula and sperm have been determined using micrococcal nuclease. The repeat length for sperm is significantly larger than blastula and gastrula repeat lengths whereas blastula and gastrula repeat lengths are not significantly different. Nucleosomal core particles have been isolated from early blastula, gastrula and sperm of sea urchins. After DNase I digestion of 51-labelled core particles the rate constants of cutting of the DNA at the susceptible sites on these core particles have been determined. The DNase I digestion kinetics of blastula and gastrula core particles are similar whereas sperm core particles are digested at a slower rate, mainly at the sites which are closest to the ends of the core particle DNA. Also, a site, which is 5 bases on the outside of the core particle and which is partially protected from nuclease attack, has been identified. The implications of these findings in relation to the histone variants in embryos and sperm of sea urchins are discussed. DA - 1983 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1983 T1 - Deoxyribonuclease probing on sea urchin embryo chromatin TI - Deoxyribonuclease probing on sea urchin embryo chromatin UR - http://hdl.handle.net/11427/17600 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/17600
dc.identifier.vancouvercitationLandsman D. Deoxyribonuclease probing on sea urchin embryo chromatin. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1983 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/17600en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBiochemistryen_ZA
dc.titleDeoxyribonuclease probing on sea urchin embryo chromatinen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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