Gene cloning studies in two nocardioform bacteria

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dc.contributor.advisorWoods, David Ren_ZA
dc.contributor.authorHill, Russellen_ZA
dc.date.accessioned2016-09-25T16:46:55Z
dc.date.available2016-09-25T16:46:55Z
dc.date.issued1988en_ZA
dc.descriptionBibliography: pages 147-177.en_ZA
dc.description.abstractNocardioforms are Gram-positive, aerobic actinomycetes and are a metabolically diverse group which produce antibiotics, useful enzymes, are important in the biotransformation of organic compounds and the decomposition of organic wastes and are important medically. A gene cloning vector designated pLR591 was constructed from the broad host range, multicopy Streptomyces plasmid pIJ702 and the positive selection Escherichia coli plasmid pEcoR251. This plasmid has useful features for the construction of actinomycete genomic libraries. Cloning of DNA into the unique Bg1II endonuclease site of pLR591 inactivated the lethal EcoRI gene derived from pEcoR251, thereby selecting for recombinant plasmids containing inserted DNA. The thiostrepton resistance gene derived from pIJ702 was shown to be functional in Streptomyces lividans enabling selection of recombinant pLR591 plasmids containing foreign DNA in S. lividans. The vector pLR591 therefore functions as a positive selection Streptomyces-E. coli shuttle vector facilitating construction of actinomycete genomic libraries in E. coli and subsequent transfer of recombinant plasmids into S. lividans.en_ZA
dc.identifier.apacitationHill, R. (1988). <i>Gene cloning studies in two nocardioform bacteria</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/21896en_ZA
dc.identifier.chicagocitationHill, Russell. <i>"Gene cloning studies in two nocardioform bacteria."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1988. http://hdl.handle.net/11427/21896en_ZA
dc.identifier.citationHill, R. 1988. Gene cloning studies in two nocardioform bacteria. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Hill, Russell AB - Nocardioforms are Gram-positive, aerobic actinomycetes and are a metabolically diverse group which produce antibiotics, useful enzymes, are important in the biotransformation of organic compounds and the decomposition of organic wastes and are important medically. A gene cloning vector designated pLR591 was constructed from the broad host range, multicopy Streptomyces plasmid pIJ702 and the positive selection Escherichia coli plasmid pEcoR251. This plasmid has useful features for the construction of actinomycete genomic libraries. Cloning of DNA into the unique Bg1II endonuclease site of pLR591 inactivated the lethal EcoRI gene derived from pEcoR251, thereby selecting for recombinant plasmids containing inserted DNA. The thiostrepton resistance gene derived from pIJ702 was shown to be functional in Streptomyces lividans enabling selection of recombinant pLR591 plasmids containing foreign DNA in S. lividans. The vector pLR591 therefore functions as a positive selection Streptomyces-E. coli shuttle vector facilitating construction of actinomycete genomic libraries in E. coli and subsequent transfer of recombinant plasmids into S. lividans. DA - 1988 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1988 T1 - Gene cloning studies in two nocardioform bacteria TI - Gene cloning studies in two nocardioform bacteria UR - http://hdl.handle.net/11427/21896 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21896
dc.identifier.vancouvercitationHill R. Gene cloning studies in two nocardioform bacteria. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1988 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21896en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherActinomycetalesen_ZA
dc.subject.otherBacterial geneticsen_ZA
dc.titleGene cloning studies in two nocardioform bacteriaen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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