The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants

Thesis / Dissertation

2003

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http://hdl.handle.net/11427/40101
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thesis_sci_2003_garwe dahlia.pdf (8.23 MB)
Authors
Garwe, Dahlia
Supervisors
Thomson, Jennifer
Mundree, Sagadevan
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Department of Molecular and Cell Biology
Faculty
Faculty of Science
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Abstract
The strategy of "complementation by functional sufficiency" was used to isolate a cDNA, designated XVSAP1, from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there are at least two copies of XVSAP1 in X. viscosa. The deduced amino acid sequence showed 49% identity to WCOR413, a low temperature-regulated protein from wheat. The protein also showed between 25% to 56% identity to WCOR413-like proteins from Arabidopsis thaliana.
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Molecular and Cell Biology

Reference:

Garwe, D. 2003. The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants. . ,Faculty of Science ,Department of Molecular and Cell Biology. http://hdl.handle.net/11427/40101

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PhD / Doctoral