Investigations of the molecular determinants of maize streak virus replication

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dc.contributor.advisorRybicki, Eden_ZA
dc.contributor.authorWillment, Janet Anneen_ZA
dc.date.accessioned2014-12-02T14:59:46Z
dc.date.available2014-12-02T14:59:46Z
dc.date.issued1999en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractGeminiviruses replicate via a rolling circle mechanism, which initiates at the origin of replication located within the long intergenic region (LIR). The viral replication associated-protein (Rep) in conjunction with the host's DNA replication machinery is responsible for the initiation and termination of the replication cycle from a stem-loop structure, located within the LIR and conserved throughout the three genera of Geminiviridae. The specific interaction between the Rep protein with sequences within the intergenic region has been well characterised for the begomoviruses and to some extent the curtoviruses; however, this interaction in the mastreviruses, and in particular maize streak virus (MSV), has yet to be fully explored. A theoretical model has been proposed based on sequence data and informed by the current understanding of replication specificity in begomoviruses. Due to the lack of conservation of the stem sequence of the stem-loop structure amongst mastreviruses, the model implicates a pair of nucleotide sequence repeats called iterons. These are located within the stem structure, and on the complementary sense side of the LIR. The former is the putative site of Rep interaction with the LIR. These iterons would therefore potentially act as the determinants of replication specificity amongst mastreviruses.en_ZA
dc.identifier.apacitationWillment, J. A. (1999). <i>Investigations of the molecular determinants of maize streak virus replication</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9869en_ZA
dc.identifier.chicagocitationWillment, Janet Anne. <i>"Investigations of the molecular determinants of maize streak virus replication."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1999. http://hdl.handle.net/11427/9869en_ZA
dc.identifier.citationWillment, J. 1999. Investigations of the molecular determinants of maize streak virus replication. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Willment, Janet Anne AB - Geminiviruses replicate via a rolling circle mechanism, which initiates at the origin of replication located within the long intergenic region (LIR). The viral replication associated-protein (Rep) in conjunction with the host's DNA replication machinery is responsible for the initiation and termination of the replication cycle from a stem-loop structure, located within the LIR and conserved throughout the three genera of Geminiviridae. The specific interaction between the Rep protein with sequences within the intergenic region has been well characterised for the begomoviruses and to some extent the curtoviruses; however, this interaction in the mastreviruses, and in particular maize streak virus (MSV), has yet to be fully explored. A theoretical model has been proposed based on sequence data and informed by the current understanding of replication specificity in begomoviruses. Due to the lack of conservation of the stem sequence of the stem-loop structure amongst mastreviruses, the model implicates a pair of nucleotide sequence repeats called iterons. These are located within the stem structure, and on the complementary sense side of the LIR. The former is the putative site of Rep interaction with the LIR. These iterons would therefore potentially act as the determinants of replication specificity amongst mastreviruses. DA - 1999 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1999 T1 - Investigations of the molecular determinants of maize streak virus replication TI - Investigations of the molecular determinants of maize streak virus replication UR - http://hdl.handle.net/11427/9869 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9869
dc.identifier.vancouvercitationWillment JA. Investigations of the molecular determinants of maize streak virus replication. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1999 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9869en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleInvestigations of the molecular determinants of maize streak virus replicationen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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