Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector

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dc.contributor.advisorChapman, Rosamunden_ZA
dc.contributor.authorGriffin, Sarahen_ZA
dc.date.accessioned2014-07-28T08:19:16Z
dc.date.available2014-07-28T08:19:16Z
dc.date.issued2007en_ZA
dc.descriptionIncludes bibliographical references (leaves 89-95).
dc.description.abstractThis study aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication factor, and the repA and repB genes which code for essential replication proteins. Specific aspects of this typical mycobacterial replicon were identified as potential targets to improve stability and increase recombinant protein expression levels, and were modified accordingly.en_ZA
dc.identifier.apacitationGriffin, S. (2007). <i>Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Virology. Retrieved from http://hdl.handle.net/11427/2727en_ZA
dc.identifier.chicagocitationGriffin, Sarah. <i>"Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Virology, 2007. http://hdl.handle.net/11427/2727en_ZA
dc.identifier.citationGriffin, S. 2007. Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Griffin, Sarah AB - This study aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication factor, and the repA and repB genes which code for essential replication proteins. Specific aspects of this typical mycobacterial replicon were identified as potential targets to improve stability and increase recombinant protein expression levels, and were modified accordingly. DA - 2007 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2007 T1 - Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector TI - Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector UR - http://hdl.handle.net/11427/2727 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/2727
dc.identifier.vancouvercitationGriffin S. Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Virology, 2007 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/2727en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Virologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMedical Virologyen_ZA
dc.titleOptimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vectoren_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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