Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector

Master Thesis

2007

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http://hdl.handle.net/11427/2727
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thesis_hsf_2007_griffin_s.pdf (17.41 MB)
Authors
Griffin, Sarah
Supervisors
Chapman, Rosamund
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University of Cape Town

Department
Division of Virology
Faculty
Faculty of Health Sciences
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Abstract
This study aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication factor, and the repA and repB genes which code for essential replication proteins. Specific aspects of this typical mycobacterial replicon were identified as potential targets to improve stability and increase recombinant protein expression levels, and were modified accordingly.
Description

Includes bibliographical references (leaves 89-95).

Keywords
Medical Virology

Reference:

Griffin, S. 2007. Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector. University of Cape Town.

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