A role of statins against listeria monocytogenes and Mycobacterium tuberculosis infection

Simple item page
dc.contributor.advisorBrombacher, Franken_ZA
dc.contributor.advisorGuler, Retoen_ZA
dc.contributor.authorParihar, Suraj Pen_ZA
dc.date.accessioned2015-10-28T05:35:18Z
dc.date.available2015-10-28T05:35:18Z
dc.date.issued2011en_ZA
dc.description.abstractCholesterol has been shown to play important role in the pathogenesis and persistence of intracellular pathogens. Here, we modulate host cholesterol biosynthesis pathway using pharmacological agent statins, which are reversible inhibitors of HMG†CoA reductase enzyme. The aim of the study was to investigate the role of statins in inducing host protective responses against intracellular pathogens. We report reduced growth of Listeria monocytogenes (LM) and Mycobacterium tuberculosis (Mtb) in murine macrophages. We show prominent immunomodulatory activity induced by statins, mainly increased phagosomal maturation and autophagy resulting in decreased bacterial growth in macrophages. Subsequently, statin†treated mice showed decrease in bacterial loads, accompanied by reduced histopathology in the acute phase of infection during listeriosis and tuberculosis. Furthermore, we found decreased growth of Mtb in peripheral blood mononuclear cells (PBMC) and monocyte†derived macrophages (MDM) isolated from patients with familial hypercholesterolemia (FH) on statin therapy when compared to healthy subjects. Together, our results show that statins induces protection against Mtb in murine macrophages, mice and human mononuclear cells and monocyte†derived macrophages.en_ZA
dc.identifier.apacitationParihar, S. P. (2011). <i>A role of statins against listeria monocytogenes and Mycobacterium tuberculosis infection</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Immunology. Retrieved from http://hdl.handle.net/11427/14393en_ZA
dc.identifier.chicagocitationParihar, Suraj P. <i>"A role of statins against listeria monocytogenes and Mycobacterium tuberculosis infection."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Immunology, 2011. http://hdl.handle.net/11427/14393en_ZA
dc.identifier.citationParihar, S. 2011. A role of statins against listeria monocytogenes and Mycobacterium tuberculosis infection. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Parihar, Suraj P AB - Cholesterol has been shown to play important role in the pathogenesis and persistence of intracellular pathogens. Here, we modulate host cholesterol biosynthesis pathway using pharmacological agent statins, which are reversible inhibitors of HMG†CoA reductase enzyme. The aim of the study was to investigate the role of statins in inducing host protective responses against intracellular pathogens. We report reduced growth of Listeria monocytogenes (LM) and Mycobacterium tuberculosis (Mtb) in murine macrophages. We show prominent immunomodulatory activity induced by statins, mainly increased phagosomal maturation and autophagy resulting in decreased bacterial growth in macrophages. Subsequently, statin†treated mice showed decrease in bacterial loads, accompanied by reduced histopathology in the acute phase of infection during listeriosis and tuberculosis. Furthermore, we found decreased growth of Mtb in peripheral blood mononuclear cells (PBMC) and monocyte†derived macrophages (MDM) isolated from patients with familial hypercholesterolemia (FH) on statin therapy when compared to healthy subjects. Together, our results show that statins induces protection against Mtb in murine macrophages, mice and human mononuclear cells and monocyte†derived macrophages. DA - 2011 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - A role of statins against listeria monocytogenes and Mycobacterium tuberculosis infection TI - A role of statins against listeria monocytogenes and Mycobacterium tuberculosis infection UR - http://hdl.handle.net/11427/14393 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14393
dc.identifier.vancouvercitationParihar SP. A role of statins against listeria monocytogenes and Mycobacterium tuberculosis infection. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Immunology, 2011 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/14393en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Immunologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherListeria monocytogenesen_ZA
dc.subject.otherMycobacterium tuberculosisen_ZA
dc.titleA role of statins against listeria monocytogenes and Mycobacterium tuberculosis infectionen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
thesis_hsf_2011_parihar_suraj_p.pdf
Size:
3.15 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
PhD / Doctoral