Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes

dc.contributor.advisorFolb, Peter Ien_ZA
dc.contributor.advisorCridland, J Sen_ZA
dc.contributor.authorHuang, Cheng-Yien_ZA
dc.date.accessioned2017-12-11T10:20:37Z
dc.date.available2017-12-11T10:20:37Z
dc.date.issued1984en_ZA
dc.description.abstractTwo separate assays for prizidilol have been developed. One relies on separation by high performance liquid chromatography, and the other on separation by thin layer chromatography. Both of these methods have several advantages over the existing assay developed by JC Pearce for Smith Kline and French Ltd. (SK & F). Firstly, both methods utilize pepsin to digest plasma protein since it is known that precipitation with trichloroacetic acid of the protein leads to losses by binding at different rates of prizidilol and of the internal standard. The SK & F assay might therefore be less sensitive and precise than necessary. Secondly, use of the complexing reagent, quinolin-3-al, leads to greater sensitivity of detection than the reagent, anisaldehyde, previously employed. The minimum level of quantitation of the developed assays are approximately 30 μg/1 by HPLC and 15 μg/1 by TLC, as against 50 μg/1 by the earlier HPLC method originating from SK & F laboratories. Finally, the TLC method is much quicker to perform than the previous assay.en_ZA
dc.identifier.apacitationHuang, C. (1984). <i>Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Clinical Pharmacology. Retrieved from http://hdl.handle.net/11427/26534en_ZA
dc.identifier.chicagocitationHuang, Cheng-Yi. <i>"Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Clinical Pharmacology, 1984. http://hdl.handle.net/11427/26534en_ZA
dc.identifier.citationHuang, C. 1984. Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Huang, Cheng-Yi AB - Two separate assays for prizidilol have been developed. One relies on separation by high performance liquid chromatography, and the other on separation by thin layer chromatography. Both of these methods have several advantages over the existing assay developed by JC Pearce for Smith Kline and French Ltd. (SK & F). Firstly, both methods utilize pepsin to digest plasma protein since it is known that precipitation with trichloroacetic acid of the protein leads to losses by binding at different rates of prizidilol and of the internal standard. The SK & F assay might therefore be less sensitive and precise than necessary. Secondly, use of the complexing reagent, quinolin-3-al, leads to greater sensitivity of detection than the reagent, anisaldehyde, previously employed. The minimum level of quantitation of the developed assays are approximately 30 μg/1 by HPLC and 15 μg/1 by TLC, as against 50 μg/1 by the earlier HPLC method originating from SK & F laboratories. Finally, the TLC method is much quicker to perform than the previous assay. DA - 1984 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1984 T1 - Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes TI - Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes UR - http://hdl.handle.net/11427/26534 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/26534
dc.identifier.vancouvercitationHuang C. Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Clinical Pharmacology, 1984 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/26534en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Clinical Pharmacologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherClinical Pharmacologyen_ZA
dc.titlePrizidilol : analytical methods and in vitro metabolism by hepatic enzymesen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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