Browsing by Author "Nembaware, Victoria Precious"

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    A formative evaluation of an instant messaging-based HIV and AIDS helpline in South Africa
    (2012) Nembaware, Victoria Precious; Goodman, Suki
    Widespread adoption of mobile phone-based innovative interventions hinge on clear guidelines and standards being established on how such programmes should be designed and implemented to promote high impact levels. This report is a formative evaluation of RedChatZone, a pilot instant messaging-based programme implemented in 2009 to provide an HIV and AIDS helpline via Mxit, an instant messaging platform.
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    Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse
    (2008) Nembaware, Victoria Precious; Seoighe, Cathal
    This dissertation aims to examine allele-specific splicing in human and mouse using publicly available datasets. Such datasets, which have been generated from multiple tissue sources and from individuals of diverse backgrounds, are rich and cheap reservoirs of transcript isoforms resulting from alternative splicing as well as isoforms resulting from mutations or polymorphisms (allele-specific isoforms). Published tools were used to analyse microarray and genomic data. However, for the assessment of allele-specific splicing using publicly available high-throughput transcript sequences, we present two novel methods: a heuristic method for quantifying the prevalence of allele-specific splicing and a more sophisticated maximum likelihood method for the detection of individual examples of allele-specific splicing. These methods make use of transcripts that can be mapped to both polymorphisms and computationally predicted mRNA isoforms. Inference of polymorphic alleles from transcripts is laborious hence a pre-computed database was created for the human data and made publicly available for use by the wider research community.