Molecular characterization of XVT8, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker.
| dc.contributor.author | Ndima, Tozama | |
| dc.contributor.author | Farrant, Jill | |
| dc.contributor.author | Thomson, Jennifer | |
| dc.contributor.author | Mundree, Sagadevan | |
| dc.date.accessioned | 2016-08-02T13:54:05Z | |
| dc.date.available | 2016-08-02T13:54:05Z | |
| dc.date.issued | 2001 | |
| dc.date.updated | 2016-08-02T09:11:10Z | |
| dc.description.abstract | Xerophyta viscosa (Baker) is a monocotyledonous resurrection plant that is capable of tolerating extremes of desiccation. Upon rewatering, it rehydrates completely, assuming its full physiological activities. Studies on changes in gene expression associated with dehydration stress tolerance were conducted. A cDNA library was constructed from mRNA isolated from dehydrated X. viscosa leaves [85%, 37% and 5% relative water content (RWC)]. XVT8 represents one of 30 randomly selected clones that were differentially expressed when X. viscosa was dehydrated. Sequence analysis of XVT8 revealed that XVT8 exhibited 45% and 43% identity to dehydrin proteins from Arabidopsis thaliana and Pisum sativum respectively, at the amino acid level. XVT8 encodes a glycine -rich protein (27 kDa) which is largely hydrophilic and contains a hydrophobic segment at the C-terminus. Southern blot analysis confirmed the presence of XVT8 in the X. viscosa genome. XVT8 transcripts accumulated in X. viscosa plants that were exposed to heat, low temperature and dehydration stresses, and to exogenous abscisic acid and ethylene. These results provide direct evidence for the heat, low temperature, dehydration, abscisic acid and ethylene -dependent regulation of the XVT8 gene in X. viscosa. | en_ZA |
| dc.identifier | http://dx.doi.org/10.1023/A:1014433821730 | |
| dc.identifier.apacitation | Ndima, T., Farrant, J., Thomson, J., & Mundree, S. (2001). Molecular characterization of XVT8, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker. <i>Plant Growth Regulation</i>, http://hdl.handle.net/11427/21102 | en_ZA |
| dc.identifier.chicagocitation | Ndima, Tozama, Jill Farrant, Jennifer Thomson, and Sagadevan Mundree "Molecular characterization of XVT8, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker." <i>Plant Growth Regulation</i> (2001) http://hdl.handle.net/11427/21102 | en_ZA |
| dc.identifier.citation | Ndima, T., Farrant, J., Thomson, J., & Mundree, S. (2001). Molecular characterization of XVT8, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker. Plant Growth Regulation, 35(2), 137-145. | en_ZA |
| dc.identifier.issn | 0167-6903 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Ndima, Tozama AU - Farrant, Jill AU - Thomson, Jennifer AU - Mundree, Sagadevan AB - Xerophyta viscosa (Baker) is a monocotyledonous resurrection plant that is capable of tolerating extremes of desiccation. Upon rewatering, it rehydrates completely, assuming its full physiological activities. Studies on changes in gene expression associated with dehydration stress tolerance were conducted. A cDNA library was constructed from mRNA isolated from dehydrated X. viscosa leaves [85%, 37% and 5% relative water content (RWC)]. XVT8 represents one of 30 randomly selected clones that were differentially expressed when X. viscosa was dehydrated. Sequence analysis of XVT8 revealed that XVT8 exhibited 45% and 43% identity to dehydrin proteins from Arabidopsis thaliana and Pisum sativum respectively, at the amino acid level. XVT8 encodes a glycine -rich protein (27 kDa) which is largely hydrophilic and contains a hydrophobic segment at the C-terminus. Southern blot analysis confirmed the presence of XVT8 in the X. viscosa genome. XVT8 transcripts accumulated in X. viscosa plants that were exposed to heat, low temperature and dehydration stresses, and to exogenous abscisic acid and ethylene. These results provide direct evidence for the heat, low temperature, dehydration, abscisic acid and ethylene -dependent regulation of the XVT8 gene in X. viscosa. DA - 2001 DB - OpenUCT DP - University of Cape Town J1 - Plant Growth Regulation LK - https://open.uct.ac.za PB - University of Cape Town PY - 2001 SM - 0167-6903 T1 - Molecular characterization of XVT8, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker TI - Molecular characterization of XVT8, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker UR - http://hdl.handle.net/11427/21102 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/21102 | |
| dc.identifier.vancouvercitation | Ndima T, Farrant J, Thomson J, Mundree S. Molecular characterization of XVT8, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker. Plant Growth Regulation. 2001; http://hdl.handle.net/11427/21102. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | Springer | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.source | Plant Growth Regulation | en_ZA |
| dc.source.uri | http://link.springer.com/journal/10725 | |
| dc.title | Molecular characterization of XVT8, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker. | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |