Tomography of asymmetric bulk specimens imaged by scanning electron microscopy

Journal Article

2010

Permanent link to this Item
http://hdl.handle.net/11427/20765
 http://www.sciencedirect.com/science/article/pii/S0304399109002447
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Woodward_Tomography_asymmetric_2010.pdf (432.73 KB)
Authors
Woodward, J D
Sewell, B T
Journal Title

Ultramicroscopy

Link to Journal
http://www.sciencedirect.com/science/article/pii/S0304399109002447
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Volume Title
Publisher

Elsevier

Publisher

University of Cape Town

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Abstract
The scanning electron microscope produces nanometer-resolution surface images of biological samples preserved in a life-like state. Extracting three-dimensional information from these two-dimensional images has been the subject of long and ongoing research. We present here a general method and theoretical basis for reconstructing the surfaces of SEM specimens imaged from multiple directions by back-projection. The resulting reconstructions are faithful representations of the original specimen geometry, even when the input images are blurred and have low signal-to-noise ratio.
Description
Keywords
Scanning electron microscopy
Conical tomography
Three-dimensional reconstruction
Back-projection

Reference:

Woodward, J. D., & Sewell, B. T. (2010). Tomography of asymmetric bulk specimens imaged by scanning electron microscopy. Ultramicroscopy, 110(2), 170-175.

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