Identification and characterization of a polyomavirus in the thornback skate (Raja clavata)

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dc.contributor.authorAbrantes, Joana
dc.contributor.authorVarsani, Arvind
dc.contributor.authorPereira, Paulo
dc.contributor.authorMaia, Catarina
dc.contributor.authorFarias, Inês
dc.contributor.authorVeríssimo, Ana
dc.contributor.authorNeves, Fabiana
dc.date.accessioned2023-09-19T08:59:58Z
dc.date.available2023-09-19T08:59:58Z
dc.date.issued2023-08-24
dc.date.updated2023-08-27T03:11:19Z
dc.description.abstractAbstract Members of the family Polyomaviridae have a circular double-stranded DNA genome that have been identified in various hosts ranging from mammals to arachnids. Here we report the identification and analysis of a complete genome sequence of a novel polyomavirus, Raja clavata polyomavirus (RcPyV1), from a cartilaginous fish, the thornback skate (Raja clavata). The genome sequence was determined using a metagenomics approach with an aim to provide baseline viral data in cartilaginous fish in different ecosystems. The RcPyV1 genome (4,195 nucleotides) had typical organization of polyomavirus, including early antigens (small T; Large T) encoded on one strand and late viral proteins (VP1; VP2) on the complementary strand. Maximum-likelihood phylogenetic analysis of the large T-antigen revealed that RcPyV1 clusters with a polyomavirus obtained from another cartilaginous fish, the guitarfish polyomavirus 1 (GfPyV1). These two share ~ 56% pairwise identity in LT and VP1 protein sequences. These analyses support the hypothesis that cartilaginous fishes have a specific lineage of polyomaviruses.
dc.identifier.apacitationAbrantes, J., Varsani, A., Pereira, P., Maia, C., Farias, I., Veríssimo, A., & Neves, F. (2023). Identification and characterization of a polyomavirus in the thornback skate (Raja clavata). <i>Virology Journal</i>, 20(1), 190. http://hdl.handle.net/11427/38765en_ZA
dc.identifier.chicagocitationAbrantes, Joana, Arvind Varsani, Paulo Pereira, Catarina Maia, Inês Farias, Ana Veríssimo, and Fabiana Neves "Identification and characterization of a polyomavirus in the thornback skate (Raja clavata)." <i>Virology Journal</i> 20, 1. (2023): 190. http://hdl.handle.net/11427/38765en_ZA
dc.identifier.citationAbrantes, J., Varsani, A., Pereira, P., Maia, C., Farias, I., Veríssimo, A. & Neves, F. 2023. Identification and characterization of a polyomavirus in the thornback skate (Raja clavata). <i>Virology Journal.</i> 20(1):190. http://hdl.handle.net/11427/38765en_ZA
dc.identifier.risTY - Journal Article AU - Abrantes, Joana AU - Varsani, Arvind AU - Pereira, Paulo AU - Maia, Catarina AU - Farias, Inês AU - Veríssimo, Ana AU - Neves, Fabiana AB - Abstract Members of the family Polyomaviridae have a circular double-stranded DNA genome that have been identified in various hosts ranging from mammals to arachnids. Here we report the identification and analysis of a complete genome sequence of a novel polyomavirus, Raja clavata polyomavirus (RcPyV1), from a cartilaginous fish, the thornback skate (Raja clavata). The genome sequence was determined using a metagenomics approach with an aim to provide baseline viral data in cartilaginous fish in different ecosystems. The RcPyV1 genome (4,195 nucleotides) had typical organization of polyomavirus, including early antigens (small T; Large T) encoded on one strand and late viral proteins (VP1; VP2) on the complementary strand. Maximum-likelihood phylogenetic analysis of the large T-antigen revealed that RcPyV1 clusters with a polyomavirus obtained from another cartilaginous fish, the guitarfish polyomavirus 1 (GfPyV1). These two share ~ 56% pairwise identity in LT and VP1 protein sequences. These analyses support the hypothesis that cartilaginous fishes have a specific lineage of polyomaviruses. DA - 2023-08-24 DB - OpenUCT DP - University of Cape Town KW - Cartilaginous fishes KW - Thornback skate KW - Polyomaviridae LK - https://open.uct.ac.za PY - 2023 T1 - Identification and characterization of a polyomavirus in the thornback skate (Raja clavata) TI - Identification and characterization of a polyomavirus in the thornback skate (Raja clavata) UR - http://hdl.handle.net/11427/38765 ER -en_ZA
dc.identifier.urihttps://doi.org/10.1186/s12985-023-02149-1
dc.identifier.urihttp://hdl.handle.net/11427/38765
dc.identifier.vancouvercitationAbrantes J, Varsani A, Pereira P, Maia C, Farias I, Veríssimo A, et al. Identification and characterization of a polyomavirus in the thornback skate (Raja clavata). Virology Journal. 2023;20(1):190. http://hdl.handle.net/11427/38765.en_ZA
dc.language.rfc3066en
dc.publisherBioMed Central
dc.publisher.departmentIntegrative Biomedical Sciences
dc.publisher.facultyHealth Sciences
dc.rights.holderBioMed Central Ltd., part of Springer Nature
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceVirology Journal
dc.source.journalissue1
dc.source.journalvolume20
dc.source.pagination190
dc.source.urihttps://virologyj.biomedcentral.com/
dc.subjectCartilaginous fishes
dc.subjectThornback skate
dc.subjectPolyomaviridae
dc.titleIdentification and characterization of a polyomavirus in the thornback skate (Raja clavata)
dc.typeJournal Article
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