An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana

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dc.contributor.advisorIsaacson, Leonen_ZA
dc.contributor.authorFisher, David Willisen_ZA
dc.date.accessioned2016-03-17T07:06:21Z
dc.date.available2016-03-17T07:06:21Z
dc.date.issued1993en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractA knowledge of the ionic and electrical gradients across the Malpighian tubules of Onymacris plana is fundamental to the understanding of the transport mechanisms of fluid across the basolateral and apical membranes. Until now no one has investigated the isolated perfused Malpighian tubule of Onymacris plana and simultaneously measured basolateral potentials. Malpighian tubule segments (about lmm length) were dissected out and perfused in vitro according to the method of Burg et. al. (1966), while the basolateral membrane potential (Vbl) was measured by means of an intracellular microelectrode. Using cable analysis, the effects of DNP, chloride free Ringers, BaCl² (a K⁺-channel blocker), low (25 mM) and high (130 mM) pntassium Ringers (bathing medium), cyclic-AMP and a corpora cardiaca homogenate (CCH) on. the transepithelial resistance and the fractional resistances of the basolateral and apical membranes were investigated.en_ZA
dc.identifier.apacitationFisher, D. W. (1993). <i>An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,MRC/UCT RU for Exercise and Sport Medicine. Retrieved from http://hdl.handle.net/11427/17861en_ZA
dc.identifier.chicagocitationFisher, David Willis. <i>"An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,MRC/UCT RU for Exercise and Sport Medicine, 1993. http://hdl.handle.net/11427/17861en_ZA
dc.identifier.citationFisher, D. 1993. An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Fisher, David Willis AB - A knowledge of the ionic and electrical gradients across the Malpighian tubules of Onymacris plana is fundamental to the understanding of the transport mechanisms of fluid across the basolateral and apical membranes. Until now no one has investigated the isolated perfused Malpighian tubule of Onymacris plana and simultaneously measured basolateral potentials. Malpighian tubule segments (about lmm length) were dissected out and perfused in vitro according to the method of Burg et. al. (1966), while the basolateral membrane potential (Vbl) was measured by means of an intracellular microelectrode. Using cable analysis, the effects of DNP, chloride free Ringers, BaCl² (a K⁺-channel blocker), low (25 mM) and high (130 mM) pntassium Ringers (bathing medium), cyclic-AMP and a corpora cardiaca homogenate (CCH) on. the transepithelial resistance and the fractional resistances of the basolateral and apical membranes were investigated. DA - 1993 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1993 T1 - An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana TI - An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana UR - http://hdl.handle.net/11427/17861 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/17861
dc.identifier.vancouvercitationFisher DW. An electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris plana. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,MRC/UCT RU for Exercise and Sport Medicine, 1993 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/17861en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentMRC/UCT RU for Exercise and Sport Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherPhysiologyen_ZA
dc.titleAn electrophysiological study of ion transport across the isolated perfused malpighian tubule of Onymacris planaen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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