Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA.
| dc.contributor.author | Koonjul, P | |
| dc.contributor.author | Brandt, W | |
| dc.contributor.author | Farrant, J | |
| dc.contributor.author | Lindsey, G | |
| dc.date.accessioned | 2016-08-01T12:59:53Z | |
| dc.date.available | 2016-08-01T12:59:53Z | |
| dc.date.issued | 1999 | |
| dc.date.updated | 2016-08-01T11:39:53Z | |
| dc.description.abstract | Polysaccharides, secondary metabolites and poly-phenolics are known to co-isolate with nucleic acids from plant tissues resulting in inhibition of molecular manipulations. RNA isolated from the polyphenolic-rich resurrection plant, Myrothamnus flabellifolius, was demonstrated to inhibit a standard polymerase chain reaction used as an assay despite the inclusion of the polyphenolic-binding compound poly(1-vinylpyrrolidone-2) (PVP) into the RNA isolation medium. This inhibition was, however, reversed by the addition of PVP into the PCR mixture itself. Confirmation of the inhibitory effect of polyphenolics on PCR was obtained by addition of green tea polyphenolics to the standard PCR assay. This inhibition was also reversed by the simultaneous inclusion of PVP. | en_ZA |
| dc.identifier.apacitation | Koonjul, P., Brandt, W., Farrant, J., & Lindsey, G. (1999). Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA. <i>Nucleic Acids Research</i>, http://hdl.handle.net/11427/21089 | en_ZA |
| dc.identifier.chicagocitation | Koonjul, P, W Brandt, J Farrant, and G Lindsey "Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA." <i>Nucleic Acids Research</i> (1999) http://hdl.handle.net/11427/21089 | en_ZA |
| dc.identifier.citation | Koonjul, P. K., Brandt, W. F., Lindsey, G. G., & Farrant, J. M. (1999). Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA. Nucleic acids research, 27(3), 915-916. | en_ZA |
| dc.identifier.issn | 0305-1048 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Koonjul, P AU - Brandt, W AU - Farrant, J AU - Lindsey, G AB - Polysaccharides, secondary metabolites and poly-phenolics are known to co-isolate with nucleic acids from plant tissues resulting in inhibition of molecular manipulations. RNA isolated from the polyphenolic-rich resurrection plant, Myrothamnus flabellifolius, was demonstrated to inhibit a standard polymerase chain reaction used as an assay despite the inclusion of the polyphenolic-binding compound poly(1-vinylpyrrolidone-2) (PVP) into the RNA isolation medium. This inhibition was, however, reversed by the addition of PVP into the PCR mixture itself. Confirmation of the inhibitory effect of polyphenolics on PCR was obtained by addition of green tea polyphenolics to the standard PCR assay. This inhibition was also reversed by the simultaneous inclusion of PVP. DA - 1999 DB - OpenUCT DP - University of Cape Town J1 - Nucleic Acids Research LK - https://open.uct.ac.za PB - University of Cape Town PY - 1999 SM - 0305-1048 T1 - Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA TI - Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA UR - http://hdl.handle.net/11427/21089 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/21089 | |
| dc.identifier.vancouvercitation | Koonjul P, Brandt W, Farrant J, Lindsey G. Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA. Nucleic Acids Research. 1999; http://hdl.handle.net/11427/21089. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | Oxford University Press | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.rights | Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | en_ZA |
| dc.source | Nucleic Acids Research | en_ZA |
| dc.source.uri | http://nar.oxfordjournals.org/ | |
| dc.title | Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA. | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |