Feed and Host Genetics Drive Microbiome Diversity with Resultant Consequences for Production Traits in Mass-Reared Black Soldier Fly (Hermetia illucens) Larvae
| dc.contributor.author | Greenwood, Matthew P | |
| dc.contributor.author | Hull, Kelvin L | |
| dc.contributor.author | Brink-Hull, Marissa | |
| dc.contributor.author | Lloyd, Melissa | |
| dc.contributor.author | Rhode, Clint | |
| dc.date.accessioned | 2022-04-03T18:49:21Z | |
| dc.date.available | 2022-04-03T18:49:21Z | |
| dc.date.issued | 2021-12-01 | |
| dc.date.updated | 2021-12-23T15:06:22Z | |
| dc.description.abstract | Mass rearing the black soldier fly, <i>Hermetia illucens</i>, for waste bioremediation and valorisation is gaining traction on a global scale. While the health and productivity of this species are underpinned by associations with microbial taxa, little is known about the factors that govern gut microbiome assembly, function, and contributions towards host phenotypic development in actively feeding larvae. In the present study, a 16S rDNA gene sequencing approach applied to a study system incorporating both feed substrate and genetic variation is used to address this knowledge gap. It is determined that the alpha diversity of larval gut bacterial communities is driven primarily by features of the larval feed substrate, including the diversity of exogenous bacterial populations. Microbiome beta diversity, however, demonstrated patterns of differentiation consistent with an influence of diet, larval genetic background, and a potential interaction between these factors. Moreover, evidence for an association between microbiome structure and the rate of larval fat accumulation was uncovered. Taxonomic enrichment analysis and clustering of putative functional gut profiles further suggested that feed-dependent turnover in microbiome communities is most likely to impact larval characteristics. Taken together, these findings indicate that host–microbiome interactions in this species are complex yet relevant to larval trait emergence. | |
| dc.identifier | doi: 10.3390/insects12121082 | |
| dc.identifier.apacitation | Greenwood, M. P., Hull, K. L., Brink-Hull, M., Lloyd, M., & Rhode, C. (2021). Feed and Host Genetics Drive Microbiome Diversity with Resultant Consequences for Production Traits in Mass-Reared Black Soldier Fly (Hermetia illucens) Larvae. <i>Insects</i>, 12(12), http://hdl.handle.net/11427/36249 | en_ZA |
| dc.identifier.chicagocitation | Greenwood, Matthew P, Kelvin L Hull, Marissa Brink-Hull, Melissa Lloyd, and Clint Rhode "Feed and Host Genetics Drive Microbiome Diversity with Resultant Consequences for Production Traits in Mass-Reared Black Soldier Fly (Hermetia illucens) Larvae." <i>Insects</i> 12, 12. (2021) http://hdl.handle.net/11427/36249 | en_ZA |
| dc.identifier.citation | Greenwood, M.P., Hull, K.L., Brink-Hull, M., Lloyd, M. & Rhode, C. 2021. Feed and Host Genetics Drive Microbiome Diversity with Resultant Consequences for Production Traits in Mass-Reared Black Soldier Fly (Hermetia illucens) Larvae. <i>Insects.</i> 12(12) http://hdl.handle.net/11427/36249 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Greenwood, Matthew P AU - Hull, Kelvin L AU - Brink-Hull, Marissa AU - Lloyd, Melissa AU - Rhode, Clint AB - Mass rearing the black soldier fly, <i>Hermetia illucens</i>, for waste bioremediation and valorisation is gaining traction on a global scale. While the health and productivity of this species are underpinned by associations with microbial taxa, little is known about the factors that govern gut microbiome assembly, function, and contributions towards host phenotypic development in actively feeding larvae. In the present study, a 16S rDNA gene sequencing approach applied to a study system incorporating both feed substrate and genetic variation is used to address this knowledge gap. It is determined that the alpha diversity of larval gut bacterial communities is driven primarily by features of the larval feed substrate, including the diversity of exogenous bacterial populations. Microbiome beta diversity, however, demonstrated patterns of differentiation consistent with an influence of diet, larval genetic background, and a potential interaction between these factors. Moreover, evidence for an association between microbiome structure and the rate of larval fat accumulation was uncovered. Taxonomic enrichment analysis and clustering of putative functional gut profiles further suggested that feed-dependent turnover in microbiome communities is most likely to impact larval characteristics. Taken together, these findings indicate that host–microbiome interactions in this species are complex yet relevant to larval trait emergence. DA - 2021-12-01 DB - OpenUCT DP - University of Cape Town IS - 12 J1 - Insects LK - https://open.uct.ac.za PY - 2021 T1 - Feed and Host Genetics Drive Microbiome Diversity with Resultant Consequences for Production Traits in Mass-Reared Black Soldier Fly (Hermetia illucens) Larvae TI - Feed and Host Genetics Drive Microbiome Diversity with Resultant Consequences for Production Traits in Mass-Reared Black Soldier Fly (Hermetia illucens) Larvae UR - http://hdl.handle.net/11427/36249 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/36249 | |
| dc.identifier.vancouvercitation | Greenwood MP, Hull KL, Brink-Hull M, Lloyd M, Rhode C. Feed and Host Genetics Drive Microbiome Diversity with Resultant Consequences for Production Traits in Mass-Reared Black Soldier Fly (Hermetia illucens) Larvae. Insects. 2021;12(12) http://hdl.handle.net/11427/36249. | en_ZA |
| dc.publisher | Multidisciplinary Digital Publishing Institute | |
| dc.source | Insects | |
| dc.source.journalissue | 12 | |
| dc.source.journalissue | 1082 | |
| dc.source.journalvolume | 12 | |
| dc.source.uri | https://www.mdpi.com/journal/insects | |
| dc.title | Feed and Host Genetics Drive Microbiome Diversity with Resultant Consequences for Production Traits in Mass-Reared Black Soldier Fly (Hermetia illucens) Larvae | |
| dc.type | Journal Article |