Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection

Simple item page
dc.contributor.authorHiller, N Luisaen_ZA
dc.contributor.authorAhmed, Azaden_ZA
dc.contributor.authorPowell, Evanen_ZA
dc.contributor.authorMartin, Darren Pen_ZA
dc.contributor.authorEutsey, Roryen_ZA
dc.contributor.authorEarl, Joshen_ZA
dc.contributor.authorJanto, Benjaminen_ZA
dc.contributor.authorBoissy, Robert Jen_ZA
dc.contributor.authorHogg, Justinen_ZA
dc.contributor.authorBarbadora, Karenen_ZA
dc.date.accessioned2015-12-28T06:47:31Z
dc.date.available2015-12-28T06:47:31Z
dc.date.issued2010en_ZA
dc.description.abstractAlthough there is tremendous interest in understanding the evolutionary roles of horizontal gene transfer (HGT) processes that occur during chronic polyclonal infections, to date there have been few studies that directly address this topic. We have characterized multiple HGT events that most likely occurred during polyclonal infection among nasopharyngeal strains of Streptococcus pneumoniae recovered from a child suffering from chronic upper respiratory and middle-ear infections. Whole genome sequencing and comparative genomics were performed on six isolates collected during symptomatic episodes over a period of seven months. From these comparisons we determined that five of the isolates were genetically highly similar and likely represented a dominant lineage. We analyzed all genic and allelic differences among all six isolates and found that all differences tended to occur within contiguous genomic blocks, suggestive of strain evolution by homologous recombination. From these analyses we identified three strains (two of which were recovered on two different occasions) that appear to have been derived sequentially, one from the next, each by multiple recombination events. We also identified a fourth strain that contains many of the genomic segments that differentiate the three highly related strains from one another, and have hypothesized that this fourth strain may have served as a donor multiple times in the evolution of the dominant strain line. The variations among the parent, daughter, and grand-daughter recombinant strains collectively cover greater than seven percent of the genome and are grouped into 23 chromosomal clusters. While capturing in vivo HGT, these data support the distributed genome hypothesis and suggest that a single competence event in pneumococci can result in the replacement of DNA at multiple non-adjacent loci.en_ZA
dc.identifier.apacitationHiller, N. L., Ahmed, A., Powell, E., Martin, D. P., Eutsey, R., Earl, J., ... Barbadora, K. (2010). Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection. <i>PLoS One</i>, http://hdl.handle.net/11427/16031en_ZA
dc.identifier.chicagocitationHiller, N Luisa, Azad Ahmed, Evan Powell, Darren P Martin, Rory Eutsey, Josh Earl, Benjamin Janto, Robert J Boissy, Justin Hogg, and Karen Barbadora "Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection." <i>PLoS One</i> (2010) http://hdl.handle.net/11427/16031en_ZA
dc.identifier.citationHiller, N. L., Ahmed, A., Powell, E., Martin, D. P., Eutsey, R., Earl, J., ... & Sampath, R. (2010). Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection. PLoS Pathog, 6(9), e1001108. doi:10.1371/journal.ppat.1001108en_ZA
dc.identifier.ris TY - Journal Article AU - Hiller, N Luisa AU - Ahmed, Azad AU - Powell, Evan AU - Martin, Darren P AU - Eutsey, Rory AU - Earl, Josh AU - Janto, Benjamin AU - Boissy, Robert J AU - Hogg, Justin AU - Barbadora, Karen AB - Although there is tremendous interest in understanding the evolutionary roles of horizontal gene transfer (HGT) processes that occur during chronic polyclonal infections, to date there have been few studies that directly address this topic. We have characterized multiple HGT events that most likely occurred during polyclonal infection among nasopharyngeal strains of Streptococcus pneumoniae recovered from a child suffering from chronic upper respiratory and middle-ear infections. Whole genome sequencing and comparative genomics were performed on six isolates collected during symptomatic episodes over a period of seven months. From these comparisons we determined that five of the isolates were genetically highly similar and likely represented a dominant lineage. We analyzed all genic and allelic differences among all six isolates and found that all differences tended to occur within contiguous genomic blocks, suggestive of strain evolution by homologous recombination. From these analyses we identified three strains (two of which were recovered on two different occasions) that appear to have been derived sequentially, one from the next, each by multiple recombination events. We also identified a fourth strain that contains many of the genomic segments that differentiate the three highly related strains from one another, and have hypothesized that this fourth strain may have served as a donor multiple times in the evolution of the dominant strain line. The variations among the parent, daughter, and grand-daughter recombinant strains collectively cover greater than seven percent of the genome and are grouped into 23 chromosomal clusters. While capturing in vivo HGT, these data support the distributed genome hypothesis and suggest that a single competence event in pneumococci can result in the replacement of DNA at multiple non-adjacent loci. DA - 2010 DB - OpenUCT DO - 10.1371/journal.ppat.1001108 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2010 T1 - Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection TI - Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection UR - http://hdl.handle.net/11427/16031 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16031
dc.identifier.urihttp://dx.doi.org/10.1371/journal.ppat.1001108
dc.identifier.vancouvercitationHiller NL, Ahmed A, Powell E, Martin DP, Eutsey R, Earl J, et al. Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection. PLoS One. 2010; http://hdl.handle.net/11427/16031.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2010 Hiller et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plospathogensen_ZA
dc.subject.otherDNA recombinationen_ZA
dc.subject.otherHorizontal gene transferen_ZA
dc.subject.otherSequence analysisen_ZA
dc.subject.otherSequence alignmenten_ZA
dc.subject.otherGenetic locien_ZA
dc.subject.otherGenome analysisen_ZA
dc.subject.otherPhylogeneticsen_ZA
dc.subject.otherRespiratory infectionsen_ZA
dc.titleGeneration of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infectionen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Hiller_Generation_of_Genic_Diversity_2010.pdf
Size:
1.46 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles