An H5N1 influenza DNA vaccine for South Africa

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dc.contributor.authorMortimer, Elizabeth
dc.contributor.authorHitzeroth, Inga I
dc.contributor.authorBuys, Amelia
dc.contributor.authorMbewana, Sandiswa
dc.contributor.authorRybicki, Edward P
dc.date.accessioned2021-10-08T07:16:06Z
dc.date.available2021-10-08T07:16:06Z
dc.date.issued2013
dc.description.abstractThe highly pathogenic avian influenza virus H5N1 is a potent pandemic threat because of its frequent transmission from birds to humans and the increasing possibility of human to human transmission. During the 2009 H1N1 pandemic it was clear that rapid influenza vaccine production is a problem worldwide. Additionally, developing countries like South Africa generally cannot produce their own influenza vaccines because the traditional egg-based vaccine production method currently employed is too lengthy and too difficult to establish. As part of an exercise aimed at exploring the feasibility of producing emergency response influenza vaccines, we investigated an experimental DNA vaccine to the H5N1 influenza virus. We focused on the virion haemagglutinin, because it elicits the primary neutralising immune response following infection. Accordingly, we developed an H5N1 DNA vaccine with full-length and truncated versions of the haemagglutinin gene, to match previously developed protein candidates. Vaccinated mice developed a strong antibody response to the haemagglutinin protein. In addition, the full-length H5 gene elicited high haemagglutination inhibition titres in mice, indicating that it has potential as a candidate pandemic vaccine for South Africa.
dc.identifier.apacitationMortimer, E., Hitzeroth, I. I., Buys, A., Mbewana, S., & Rybicki, E. P. (2013). An H5N1 influenza DNA vaccine for South Africa. <i>South African Journal of Science</i>, 109(43353), 1 - 4. http://hdl.handle.net/11427/34770en_ZA
dc.identifier.chicagocitationMortimer, Elizabeth, Inga I Hitzeroth, Amelia Buys, Sandiswa Mbewana, and Edward P Rybicki "An H5N1 influenza DNA vaccine for South Africa." <i>South African Journal of Science</i> 109, 43353. (2013): 1 - 4. http://hdl.handle.net/11427/34770en_ZA
dc.identifier.citationMortimer, E., Hitzeroth, I.I., Buys, A., Mbewana, S. & Rybicki, E.P. 2013. An H5N1 influenza DNA vaccine for South Africa. <i>South African Journal of Science.</i> 109(43353):1 - 4. http://hdl.handle.net/11427/34770en_ZA
dc.identifier.issn0038-2353
dc.identifier.issn1996-7489
dc.identifier.ris TY - Journal Article AU - Mortimer, Elizabeth AU - Hitzeroth, Inga I AU - Buys, Amelia AU - Mbewana, Sandiswa AU - Rybicki, Edward P AB - The highly pathogenic avian influenza virus H5N1 is a potent pandemic threat because of its frequent transmission from birds to humans and the increasing possibility of human to human transmission. During the 2009 H1N1 pandemic it was clear that rapid influenza vaccine production is a problem worldwide. Additionally, developing countries like South Africa generally cannot produce their own influenza vaccines because the traditional egg-based vaccine production method currently employed is too lengthy and too difficult to establish. As part of an exercise aimed at exploring the feasibility of producing emergency response influenza vaccines, we investigated an experimental DNA vaccine to the H5N1 influenza virus. We focused on the virion haemagglutinin, because it elicits the primary neutralising immune response following infection. Accordingly, we developed an H5N1 DNA vaccine with full-length and truncated versions of the haemagglutinin gene, to match previously developed protein candidates. Vaccinated mice developed a strong antibody response to the haemagglutinin protein. In addition, the full-length H5 gene elicited high haemagglutination inhibition titres in mice, indicating that it has potential as a candidate pandemic vaccine for South Africa. DA - 2013 DB - OpenUCT DP - University of Cape Town IS - 43353 J1 - South African Journal of Science LK - https://open.uct.ac.za PY - 2013 SM - 0038-2353 SM - 1996-7489 T1 - An H5N1 influenza DNA vaccine for South Africa TI - An H5N1 influenza DNA vaccine for South Africa UR - http://hdl.handle.net/11427/34770 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/34770
dc.identifier.vancouvercitationMortimer E, Hitzeroth II, Buys A, Mbewana S, Rybicki EP. An H5N1 influenza DNA vaccine for South Africa. South African Journal of Science. 2013;109(43353):1 - 4. http://hdl.handle.net/11427/34770.en_ZA
dc.language.isoeng
dc.publisher.departmentDepartment of Molecular and Cell Biology
dc.publisher.facultyFaculty of Science
dc.sourceSouth African Journal of Science
dc.source.journalissue43353
dc.source.journalvolume109
dc.source.pagination1 - 4
dc.source.urihttps://dx.doi.org/10.1590/sajs.2013/20120053
dc.subject.otherDNA vaccine
dc.subject.otherhighly pathogenic avian influenza virus
dc.subject.otherH5N1
dc.subject.otherhaemagglutinin
dc.titleAn H5N1 influenza DNA vaccine for South Africa
dc.typeJournal Article
uct.type.publicationResearch
uct.type.resourceJournal Article
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