Characterization of methicillin-susceptible and -resistant staphylococci in the clinical setting: a multicentre study in Nigeria

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dc.contributor.authorShittu, Adebayoen_ZA
dc.contributor.authorOyedara, Omotayoen_ZA
dc.contributor.authorAbegunrin, Fadekemien_ZA
dc.contributor.authorOkon, Kennethen_ZA
dc.contributor.authorRaji, Adeolaen_ZA
dc.contributor.authorTaiwo, Samuelen_ZA
dc.contributor.authorOgunsola, Folasadeen_ZA
dc.contributor.authorOnyedibe, Kennethen_ZA
dc.contributor.authorElisha, Gayen_ZA
dc.date.accessioned2015-11-27T09:32:16Z
dc.date.available2015-11-27T09:32:16Z
dc.date.issued2012en_ZA
dc.description.abstractBACKGROUND: The staphylococci are implicated in a variety of human infections; however, many clinical microbiology laboratories in Nigeria do not identify staphylococci (in particular coagulase negative staphylococci - CNS) to the species level. Moreover, data from multi-centre assessment on antibiotic resistance and epidemiology of the staphylococci are not available in Nigeria. This study investigated 91 non-duplicate staphylococcal isolates obtained from the microbiology laboratories of eight hospitals in Nigeria during the period January to April 2010. METHODS: Identification and antibiotic susceptibility testing was performed using the VITEK 2 system, detection of resistance genes by PCR, and molecular characterization was determined by SCCmec typing, spa and multilocus sequence typing (MLST). RESULTS: All the isolates were susceptible to mupirocin, tigecycline, vancomycin and linezolid, but 72.5% of CNS and 82.3% of Staphylococcus aureus were resistant to cotrimoxazole, while multiresistance was observed in 37 of the 40 CNS isolates. Untypeable SCCmec types (ccrC/Class A mec and ccr-negative/Class C2 mec gene complex) in two methicillin-resistant S. aureus (MRSA) were identified. Additionally, ccr-negative/Class A mec and ccr type 4/Class C2 mec gene complex was detected in one isolate each of S. sciuri and S. haemolyticus, respectively. The S. aureus isolates were classified into 21 spa types including two new types (t8987, t9008) among the methicillin-susceptible S. aureus (MSSA) isolates. Two (CC8-SCCmecnon-typeable and CC88-SCCmec IV) and four (CC8-SCCmec III/IV/V; CC30-SCCmec II/III; CC88-SCCmec IV; and ST152-SCCmecnon-typeable) MRSA clones were identified in Maiduguri (North-East Nigeria) and South-West Nigeria, respectively. The proportion of Panton-Valentine leukocidin (PVL)-positive MSSA was high (44.4%) and 56.3% of these strains were associated with sequence type (ST) 152. CONCLUSIONS: The identification of multiresistant mecA positive S. haemolyticus and S. sciuri from clinical samples indicates that characterization of CNS is important in providing information on their diversity and importance in Nigeria. There is the need to develop new SCCmec classification methods for non-typeable methicillin-resistant staphylococci, and to curtail the spread and establishment of the S. aureus ST152 clone in Nigeria. The study presents the first report of a PVL-positive ST152-SCCmecnontypeable MRSA and SCCmec typing of methicillin-resistant CNS in Nigeria.en_ZA
dc.identifier.apacitationShittu, A., Oyedara, O., Abegunrin, F., Okon, K., Raji, A., Taiwo, S., ... Elisha, G. (2012). Characterization of methicillin-susceptible and -resistant staphylococci in the clinical setting: a multicentre study in Nigeria. <i>BMC Infectious Diseases</i>, http://hdl.handle.net/11427/15389en_ZA
dc.identifier.chicagocitationShittu, Adebayo, Omotayo Oyedara, Fadekemi Abegunrin, Kenneth Okon, Adeola Raji, Samuel Taiwo, Folasade Ogunsola, Kenneth Onyedibe, and Gay Elisha "Characterization of methicillin-susceptible and -resistant staphylococci in the clinical setting: a multicentre study in Nigeria." <i>BMC Infectious Diseases</i> (2012) http://hdl.handle.net/11427/15389en_ZA
dc.identifier.citationShittu, A., Oyedara, O., Abegunrin, F., Okon, K., Raji, A., Taiwo, S., ... & Elisha, G. (2012). Characterization of methicillin-susceptible and-resistant staphylococci in the clinical setting: a multicentre study in Nigeria. BMC infectious diseases, 12(1), 286.en_ZA
dc.identifier.ris TY - Journal Article AU - Shittu, Adebayo AU - Oyedara, Omotayo AU - Abegunrin, Fadekemi AU - Okon, Kenneth AU - Raji, Adeola AU - Taiwo, Samuel AU - Ogunsola, Folasade AU - Onyedibe, Kenneth AU - Elisha, Gay AB - BACKGROUND: The staphylococci are implicated in a variety of human infections; however, many clinical microbiology laboratories in Nigeria do not identify staphylococci (in particular coagulase negative staphylococci - CNS) to the species level. Moreover, data from multi-centre assessment on antibiotic resistance and epidemiology of the staphylococci are not available in Nigeria. This study investigated 91 non-duplicate staphylococcal isolates obtained from the microbiology laboratories of eight hospitals in Nigeria during the period January to April 2010. METHODS: Identification and antibiotic susceptibility testing was performed using the VITEK 2 system, detection of resistance genes by PCR, and molecular characterization was determined by SCCmec typing, spa and multilocus sequence typing (MLST). RESULTS: All the isolates were susceptible to mupirocin, tigecycline, vancomycin and linezolid, but 72.5% of CNS and 82.3% of Staphylococcus aureus were resistant to cotrimoxazole, while multiresistance was observed in 37 of the 40 CNS isolates. Untypeable SCCmec types (ccrC/Class A mec and ccr-negative/Class C2 mec gene complex) in two methicillin-resistant S. aureus (MRSA) were identified. Additionally, ccr-negative/Class A mec and ccr type 4/Class C2 mec gene complex was detected in one isolate each of S. sciuri and S. haemolyticus, respectively. The S. aureus isolates were classified into 21 spa types including two new types (t8987, t9008) among the methicillin-susceptible S. aureus (MSSA) isolates. Two (CC8-SCCmecnon-typeable and CC88-SCCmec IV) and four (CC8-SCCmec III/IV/V; CC30-SCCmec II/III; CC88-SCCmec IV; and ST152-SCCmecnon-typeable) MRSA clones were identified in Maiduguri (North-East Nigeria) and South-West Nigeria, respectively. The proportion of Panton-Valentine leukocidin (PVL)-positive MSSA was high (44.4%) and 56.3% of these strains were associated with sequence type (ST) 152. CONCLUSIONS: The identification of multiresistant mecA positive S. haemolyticus and S. sciuri from clinical samples indicates that characterization of CNS is important in providing information on their diversity and importance in Nigeria. There is the need to develop new SCCmec classification methods for non-typeable methicillin-resistant staphylococci, and to curtail the spread and establishment of the S. aureus ST152 clone in Nigeria. The study presents the first report of a PVL-positive ST152-SCCmecnontypeable MRSA and SCCmec typing of methicillin-resistant CNS in Nigeria. DA - 2012 DB - OpenUCT DO - 10.1186/1471-2334-12-286 DP - University of Cape Town J1 - BMC Infectious Diseases LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - Characterization of methicillin-susceptible and -resistant staphylococci in the clinical setting: a multicentre study in Nigeria TI - Characterization of methicillin-susceptible and -resistant staphylococci in the clinical setting: a multicentre study in Nigeria UR - http://hdl.handle.net/11427/15389 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15389
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2334-12-286
dc.identifier.vancouvercitationShittu A, Oyedara O, Abegunrin F, Okon K, Raji A, Taiwo S, et al. Characterization of methicillin-susceptible and -resistant staphylococci in the clinical setting: a multicentre study in Nigeria. BMC Infectious Diseases. 2012; http://hdl.handle.net/11427/15389.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDivision of Medical Biochemistryen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2012 Shittu et al.; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceBMC Infectious Diseasesen_ZA
dc.source.urihttp://www.biomedcentral.com/bmcinfectdis/en_ZA
dc.subject.otherCoagulase negative staphylococcien_ZA
dc.subject.otherStaphylococcus aureusen_ZA
dc.subject.otherMultiresistanceen_ZA
dc.subject.othermecA geneen_ZA
dc.subject.otherStaphylococcus haemolyticusen_ZA
dc.subject.otherStaphylococcus sciurien_ZA
dc.subject.otherPanton Valentine Leukocidinen_ZA
dc.subject.otherSCCmec typingen_ZA
dc.subject.otherST152en_ZA
dc.subject.otherNigeriaen_ZA
dc.titleCharacterization of methicillin-susceptible and -resistant staphylococci in the clinical setting: a multicentre study in Nigeriaen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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