Investigation and visualisation of microbial attachment trends to sulphide minerals in a bioleach environment

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dc.contributor.authorAfrica, C-J, van Hille, R P
dc.contributor.authorHarrison, S T L
dc.date.accessioned2016-08-25T13:44:58Z
dc.date.available2016-08-25T13:44:58Z
dc.date.issued2009
dc.date.updated2016-08-25T13:43:03Z
dc.description.abstractTwo novel experimental approaches have been developed to investigate the attachment of microorganisms to sulphide minerals as a function of the mineral and microbial phase in a joint project with BHP Billiton, conceptualised in 2005 and initiated in 2006. In the first approach, selective attachment of pure cultures to minerals was studied in the “particle coated column reactor” using A. ferrooxidans and L. ferriphilum. The saturated reactor containing glass beads coated with fine mineral concentrates provided a quantifiable surface area of mineral concentrate and maintained good fluid flow. Results are reported for chalcopyrite and pyrite concentrates, a low grade chalcopyrite ore and quartzite. The latter, representing typical gangue material, is used as a control. A. ferrooxidans displayed greater attachment to pyrite, and selective attachment to sulfide minerals over quartz. Similar attachment behaviour trends resulted for Leptospirillum spp. In the second approach, a novel technique was developed to investigate microbial ecology of microbe-mineral attachment, site and mineral specific associations of microorganisms and spatial organisation of microbial communities present. Qualitative assessment and visualisation of microorganisms associated with the mineral surface and subsequent biofilm development was shown in the biofilm reactor, using microscopy techniques and fluorochromes. FISH analyses of A. ferrooxidans and L. ferriphilum on massive chalcopyrite sections are presented. The consequence of the observed attachment on heap bioleach performance is discussed.en_ZA
dc.identifier.apacitationAfrica, C., & Harrison, S. T. L. (2009). Investigation and visualisation of microbial attachment trends to sulphide minerals in a bioleach environment. <i>Advanced Materials Research</i>, http://hdl.handle.net/11427/21554en_ZA
dc.identifier.chicagocitationAfrica, C-J, and S T L Harrison "Investigation and visualisation of microbial attachment trends to sulphide minerals in a bioleach environment." <i>Advanced Materials Research</i> (2009) http://hdl.handle.net/11427/21554en_ZA
dc.identifier.citationAfrica, C. J., van Hille, R. P., & Harrison, S. T. (2009). Investigation and visualisation of microbial attachment trends to sulphide minerals in a bioleach environment. In Advanced Materials Research (Vol. 71, pp. 345-348). Trans Tech Publications.en_ZA
dc.identifier.ris TY - Journal Article AU - Africa, C-J, van Hille, R P AU - Harrison, S T L AB - Two novel experimental approaches have been developed to investigate the attachment of microorganisms to sulphide minerals as a function of the mineral and microbial phase in a joint project with BHP Billiton, conceptualised in 2005 and initiated in 2006. In the first approach, selective attachment of pure cultures to minerals was studied in the “particle coated column reactor” using A. ferrooxidans and L. ferriphilum. The saturated reactor containing glass beads coated with fine mineral concentrates provided a quantifiable surface area of mineral concentrate and maintained good fluid flow. Results are reported for chalcopyrite and pyrite concentrates, a low grade chalcopyrite ore and quartzite. The latter, representing typical gangue material, is used as a control. A. ferrooxidans displayed greater attachment to pyrite, and selective attachment to sulfide minerals over quartz. Similar attachment behaviour trends resulted for Leptospirillum spp. In the second approach, a novel technique was developed to investigate microbial ecology of microbe-mineral attachment, site and mineral specific associations of microorganisms and spatial organisation of microbial communities present. Qualitative assessment and visualisation of microorganisms associated with the mineral surface and subsequent biofilm development was shown in the biofilm reactor, using microscopy techniques and fluorochromes. FISH analyses of A. ferrooxidans and L. ferriphilum on massive chalcopyrite sections are presented. The consequence of the observed attachment on heap bioleach performance is discussed. DA - 2009 DB - OpenUCT DP - University of Cape Town J1 - Advanced Materials Research LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 T1 - Investigation and visualisation of microbial attachment trends to sulphide minerals in a bioleach environment TI - Investigation and visualisation of microbial attachment trends to sulphide minerals in a bioleach environment UR - http://hdl.handle.net/11427/21554 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21554
dc.identifier.urihttp://www.scientific.net/AMR.71-73.345
dc.identifier.vancouvercitationAfrica C, Harrison STL. Investigation and visualisation of microbial attachment trends to sulphide minerals in a bioleach environment. Advanced Materials Research. 2009; http://hdl.handle.net/11427/21554.en_ZA
dc.languageengen_ZA
dc.publisherTrans Tech Publicationsen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceAdvanced Materials Researchen_ZA
dc.subject.otherAt. ferrooxidans, L. ferriphilum, Microbial Attachment, Mineral Bioleaching, Sulphide Minerals
dc.titleInvestigation and visualisation of microbial attachment trends to sulphide minerals in a bioleach environmenten_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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