The plasticisation of polyhydroxybutyrate in vivo

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dc.contributor.authorHarrison, Susan T L
dc.contributor.authorChase, Howard A
dc.contributor.authorAmor, Stuart R
dc.contributor.authorBonthrone, Karen M
dc.contributor.authorSanders, Jeremy K M
dc.date.accessioned2016-08-19T11:07:27Z
dc.date.available2016-08-19T11:07:27Z
dc.date.issued1992
dc.date.updated2016-08-18T13:53:01Z
dc.description.abstractThe influence of a variety of treatments on the mobility and crystallinity of poly(hydroxybutyrate) (PHB) in whole cells and native granules has been proved using 13C-n.m.r. spectroscopy and X-ray powder diffraction, and correlated with the known biological effects of these treatments. It was concluded that at least water is responsible for PHB plasticization in vivo, and that only native mobile PHB is susceptible to depolymerases. Another, probably hydrophobic, component appears to be involved either as plasticizer or nucleation inhibitor. Three states of the granule are identified in addition to the native, biologically-competent state: freeze-drying of whole cells leads to a partially-immobilized amorphous state which can be restored virtually to native mobility by rehydration; extended centrifugation of native granules in aqueous suspension, or treatment with hydrophobic detergents under certain conditions, leads to a crystalline state that is less susceptible to exogenous depolymerase; and heating to 95 degrees C or refrigeration has no detectable effect on mobility but leads to inactivation of the granule, presumably via damage to superficial membrane or protein.en_ZA
dc.identifierhttp://dx.doi.org/10.1016/S0141-8130(05)80020-0
dc.identifier.apacitationHarrison, S. T. L., Chase, H. A., Amor, S. R., Bonthrone, K. M., & Sanders, J. K. M. (1992). The plasticisation of polyhydroxybutyrate in vivo. <i>International Journal of Biological Macromolecules</i>, http://hdl.handle.net/11427/21352en_ZA
dc.identifier.chicagocitationHarrison, Susan T L, Howard A Chase, Stuart R Amor, Karen M Bonthrone, and Jeremy K M Sanders "The plasticisation of polyhydroxybutyrate in vivo." <i>International Journal of Biological Macromolecules</i> (1992) http://hdl.handle.net/11427/21352en_ZA
dc.identifier.citationHarrison, S. T., Chase, H. A., Amor, S. R., Bonthrone, K. M., & Sanders, J. K. (1992). Plasticization of poly (hydroxybutyrate) in vivo. International journal of biological macromolecules, 14(1), 50-56.en_ZA
dc.identifier.issn0141-8130en_ZA
dc.identifier.ris TY - Journal Article AU - Harrison, Susan T L AU - Chase, Howard A AU - Amor, Stuart R AU - Bonthrone, Karen M AU - Sanders, Jeremy K M AB - The influence of a variety of treatments on the mobility and crystallinity of poly(hydroxybutyrate) (PHB) in whole cells and native granules has been proved using 13C-n.m.r. spectroscopy and X-ray powder diffraction, and correlated with the known biological effects of these treatments. It was concluded that at least water is responsible for PHB plasticization in vivo, and that only native mobile PHB is susceptible to depolymerases. Another, probably hydrophobic, component appears to be involved either as plasticizer or nucleation inhibitor. Three states of the granule are identified in addition to the native, biologically-competent state: freeze-drying of whole cells leads to a partially-immobilized amorphous state which can be restored virtually to native mobility by rehydration; extended centrifugation of native granules in aqueous suspension, or treatment with hydrophobic detergents under certain conditions, leads to a crystalline state that is less susceptible to exogenous depolymerase; and heating to 95 degrees C or refrigeration has no detectable effect on mobility but leads to inactivation of the granule, presumably via damage to superficial membrane or protein. DA - 1992 DB - OpenUCT DP - University of Cape Town J1 - International Journal of Biological Macromolecules LK - https://open.uct.ac.za PB - University of Cape Town PY - 1992 SM - 0141-8130 T1 - The plasticisation of polyhydroxybutyrate in vivo TI - The plasticisation of polyhydroxybutyrate in vivo UR - http://hdl.handle.net/11427/21352 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21352
dc.identifier.vancouvercitationHarrison STL, Chase HA, Amor SR, Bonthrone KM, Sanders JKM. The plasticisation of polyhydroxybutyrate in vivo. International Journal of Biological Macromolecules. 1992; http://hdl.handle.net/11427/21352.en_ZA
dc.languageengen_ZA
dc.publisherElsevieren_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_ZA
dc.sourceInternational Journal of Biological Macromoleculesen_ZA
dc.source.urihttp://www.sciencedirect.com/science/journal/01418130
dc.subject.otherGranule structure
dc.subject.othern.m.r.
dc.subject.otherPlasticization
dc.subject.otherPoly(hydroxybutyrate)
dc.subject.otherX-ray diffraction
dc.titleThe plasticisation of polyhydroxybutyrate in vivoen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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