Force-extension of the Amylose Polysaccharide

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dc.contributor.authorvan den Berg, Rudolfen_ZA
dc.date.accessioned2014-08-13T19:25:47Z
dc.date.available2014-08-13T19:25:47Z
dc.date.issued2009en_ZA
dc.description.abstractMyasthenia gravis (MG) is an autoimmune disorder in which auto-antibodies directed at the acetylcholine receptors (AChR) of the neuromuscular junction (NMJ) block, alter or destroy their targets. The anti-AChR antibodies cause activation of the classical complement pathway leading to inflammatory injury at the NMJ. Decay Accelerating Factor (DAF), a member of complement regulatory proteins, prevents activation of autologous components of complement pathways. The absence of DAF, in knock-out mouse models, has been shown to significantly increase the susceptibility to experimental autoimmune MG. A previous study showed that a high proportion of South African MG patients of African genetic ancestry develop immunosuppressive therapy-resistant extraocular muscle (EOM) dysfunction. We hypothesized that these patients have deficient DAF expression in their EOMs resulting in less protection from complement injury. Sequence analysis of relevant regions of the DAF gene revealed a single nucleotide polymorphism (SNP), c.-198C>G, in the promoter region in MG patients of African genetic ancestry with severe EOM MG involvement (MG n=101; Control n= 132; Odds ratio= 6.6; p=0.009). DAF-luciferase reporter assays, using 3 different cell lines (COS-7, HT1080 and C2C12) revealed that the c.-198C>G SNP (Mut-DAF) led to an increase in DAF promoter activity (en_ZA
dc.identifier.apacitationvan den Berg, R. (2009). <i>Force-extension of the Amylose Polysaccharide</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Computer Science. Retrieved from http://hdl.handle.net/11427/6372en_ZA
dc.identifier.chicagocitationvan den Berg, Rudolf. <i>"Force-extension of the Amylose Polysaccharide."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Computer Science, 2009. http://hdl.handle.net/11427/6372en_ZA
dc.identifier.citationvan den Berg, R. 2009. Force-extension of the Amylose Polysaccharide. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - van den Berg, Rudolf AB - Myasthenia gravis (MG) is an autoimmune disorder in which auto-antibodies directed at the acetylcholine receptors (AChR) of the neuromuscular junction (NMJ) block, alter or destroy their targets. The anti-AChR antibodies cause activation of the classical complement pathway leading to inflammatory injury at the NMJ. Decay Accelerating Factor (DAF), a member of complement regulatory proteins, prevents activation of autologous components of complement pathways. The absence of DAF, in knock-out mouse models, has been shown to significantly increase the susceptibility to experimental autoimmune MG. A previous study showed that a high proportion of South African MG patients of African genetic ancestry develop immunosuppressive therapy-resistant extraocular muscle (EOM) dysfunction. We hypothesized that these patients have deficient DAF expression in their EOMs resulting in less protection from complement injury. Sequence analysis of relevant regions of the DAF gene revealed a single nucleotide polymorphism (SNP), c.-198C>G, in the promoter region in MG patients of African genetic ancestry with severe EOM MG involvement (MG n=101; Control n= 132; Odds ratio= 6.6; p=0.009). DAF-luciferase reporter assays, using 3 different cell lines (COS-7, HT1080 and C2C12) revealed that the c.-198C>G SNP (Mut-DAF) led to an increase in DAF promoter activity ( DA - 2009 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 T1 - Force-extension of the Amylose Polysaccharide TI - Force-extension of the Amylose Polysaccharide UR - http://hdl.handle.net/11427/6372 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/6372
dc.identifier.vancouvercitationvan den Berg R. Force-extension of the Amylose Polysaccharide. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Computer Science, 2009 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/6372en_ZA
dc.language.isoeng
dc.publisher.departmentDepartment of Computer Scienceen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherInformation Technologyen_ZA
dc.titleForce-extension of the Amylose Polysaccharideen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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