The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat.

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dc.contributor.authorDent, Kyle C
dc.contributor.authorWeber, Brandon W
dc.contributor.authorBenedik, Michael J
dc.contributor.authorSewell, Trevor B
dc.date.accessioned2016-07-21T15:38:55Z
dc.date.available2016-07-21T15:38:55Z
dc.date.issued2009
dc.date.updated2016-07-21T15:37:37Z
dc.description.abstractThe fungal cyanide hydratases form a functionally specialized subset of the nitrilases which catalyze the hydrolysis of cyanide to formamide with high specificity. These hold great promise for the bioremediation of cyanide wastes. The low resolution (3.0 nm) three-dimensional reconstruction of negatively stained recombinant cyanide hydratase fibers from the saprophytic fungus Neurospora crassa by iterative helical real space reconstruction reveals that enzyme fibers display left-handed D1 S5.4 symmetry with a helical rise of 1.36 nm. This arrangement differs from previously characterized microbial nitrilases which demonstrate a structure built along similar principles but with a reduced helical twist. The cyanide hydratase assembly is stabilized by two dyadic interactions between dimers across the one-start helical groove. Docking of a homology-derived atomic model into the experimentally determined negative stain envelope suggests the location of charged residues which may form salt bridges and stabilize the helixen_ZA
dc.identifierhttp://dx.doi.org/10.1007/s00253-008-1735-4
dc.identifier.apacitationDent, K. C., Weber, B. W., Benedik, M. J., & Sewell, T. B. (2009). The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat. <i>Applied Microbiology and Biotechnology</i>, http://hdl.handle.net/11427/20590en_ZA
dc.identifier.chicagocitationDent, Kyle C, Brandon W Weber, Michael J Benedik, and Trevor B Sewell "The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat." <i>Applied Microbiology and Biotechnology</i> (2009) http://hdl.handle.net/11427/20590en_ZA
dc.identifier.citationDent, K. C., Weber, B. W., Benedik, M. J., & Sewell, B. T. (2009). The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat. Applied microbiology and biotechnology, 82(2), 271-278.en_ZA
dc.identifier.issn0175-7598en_ZA
dc.identifier.ris TY - Journal Article AU - Dent, Kyle C AU - Weber, Brandon W AU - Benedik, Michael J AU - Sewell, Trevor B AB - The fungal cyanide hydratases form a functionally specialized subset of the nitrilases which catalyze the hydrolysis of cyanide to formamide with high specificity. These hold great promise for the bioremediation of cyanide wastes. The low resolution (3.0 nm) three-dimensional reconstruction of negatively stained recombinant cyanide hydratase fibers from the saprophytic fungus Neurospora crassa by iterative helical real space reconstruction reveals that enzyme fibers display left-handed D1 S5.4 symmetry with a helical rise of 1.36 nm. This arrangement differs from previously characterized microbial nitrilases which demonstrate a structure built along similar principles but with a reduced helical twist. The cyanide hydratase assembly is stabilized by two dyadic interactions between dimers across the one-start helical groove. Docking of a homology-derived atomic model into the experimentally determined negative stain envelope suggests the location of charged residues which may form salt bridges and stabilize the helix DA - 2009 DB - OpenUCT DP - University of Cape Town J1 - Applied Microbiology and Biotechnology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 SM - 0175-7598 T1 - The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat TI - The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat UR - http://hdl.handle.net/11427/20590 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20590
dc.identifier.urihttp://link.springer.com/article/10.1007/s00253-008-1735-4
dc.identifier.vancouvercitationDent KC, Weber BW, Benedik MJ, Sewell TB. The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat. Applied Microbiology and Biotechnology. 2009; http://hdl.handle.net/11427/20590.en_ZA
dc.languageengen_ZA
dc.publisherSpringer Verlagen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceApplied Microbiology and Biotechnologyen_ZA
dc.source.urihttp://link.springer.com/journal/253
dc.subject.otherCyanide
dc.subject.otherNitrilase
dc.subject.other3d protein reconstruction
dc.subject.otherCyanide hydratase
dc.titleThe cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat.en_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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