Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae

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dc.contributor.advisorLindsey, George
dc.contributor.authorRobinson, Evan J
dc.date.accessioned2024-04-17T14:02:18Z
dc.date.available2024-04-17T14:02:18Z
dc.date.issued2008
dc.date.updated2024-04-17T13:29:12Z
dc.description.abstractDiminishing oil reserves are causing scientists to explore renewable and sustainable replacement fuel sources. One candidate is plant biomass, although without modification its energy-rich sugars remain unavailable to the standard industrial yeast Saccharomyces cerevisiae for fermentation to the fuel ethanol. In this study, three xylose isomerase genes originating from Haemophilus influenzae Rd K20, Arabidopsis thaliana and Bacteroides thetaiotaomicron VPI-5482 were expressed in S. cerevisiae to produce a strain capable of metabolising D-xylose (a biomass constituent). Resulting strains were analysed for protein production, growth differences and xylose isomerisation but no introduced characteristics were detected. RT-PCR suggested transcription occurred for all the genes tested but no recombinant protein nor any xylose isomerase activity was detected. An in silico bioinformatic analysis showed a putative inhibitory stem-loop structure in the mRNA containing the B. thetaiotaomicron gene which may have reduced translation. Otherwise non-functional folding or undetectable activity were concluded as the results of the expression for all genes.
dc.identifier.apacitationRobinson, E. J. (2008). <i>Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae</i>. (). ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/39405en_ZA
dc.identifier.chicagocitationRobinson, Evan J. <i>"Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae."</i> ., ,Faculty of Science ,Department of Molecular and Cell Biology, 2008. http://hdl.handle.net/11427/39405en_ZA
dc.identifier.citationRobinson, E.J. 2008. Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae. . ,Faculty of Science ,Department of Molecular and Cell Biology. http://hdl.handle.net/11427/39405en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Robinson, Evan J AB - Diminishing oil reserves are causing scientists to explore renewable and sustainable replacement fuel sources. One candidate is plant biomass, although without modification its energy-rich sugars remain unavailable to the standard industrial yeast Saccharomyces cerevisiae for fermentation to the fuel ethanol. In this study, three xylose isomerase genes originating from Haemophilus influenzae Rd K20, Arabidopsis thaliana and Bacteroides thetaiotaomicron VPI-5482 were expressed in S. cerevisiae to produce a strain capable of metabolising D-xylose (a biomass constituent). Resulting strains were analysed for protein production, growth differences and xylose isomerisation but no introduced characteristics were detected. RT-PCR suggested transcription occurred for all the genes tested but no recombinant protein nor any xylose isomerase activity was detected. An in silico bioinformatic analysis showed a putative inhibitory stem-loop structure in the mRNA containing the B. thetaiotaomicron gene which may have reduced translation. Otherwise non-functional folding or undetectable activity were concluded as the results of the expression for all genes. DA - 2008 DB - OpenUCT DP - University of Cape Town KW - Molecular and Cell Biology LK - https://open.uct.ac.za PY - 2008 T1 - Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae TI - Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae UR - http://hdl.handle.net/11427/39405 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/39405
dc.identifier.vancouvercitationRobinson EJ. Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae. []. ,Faculty of Science ,Department of Molecular and Cell Biology, 2008 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/39405en_ZA
dc.language.rfc3066eng
dc.publisher.departmentDepartment of Molecular and Cell Biology
dc.publisher.facultyFaculty of Science
dc.subjectMolecular and Cell Biology
dc.titleEvaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae
dc.typeThesis / Dissertation
dc.type.qualificationlevelMasters
dc.type.qualificationlevelMSc
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