Comprehensive Cardiovascular magnetic resonance of myocardial mechanics in mice using three-dimensional cine DENSE

Simple item page
dc.contributor.authorZhong, Xiaodongen_ZA
dc.contributor.authorGibberman, Laurenen_ZA
dc.contributor.authorSpottiswoode, Bruceen_ZA
dc.contributor.authorGilliam, Andrewen_ZA
dc.contributor.authorMeyer, Craigen_ZA
dc.contributor.authorFrench, Brenten_ZA
dc.contributor.authorEpstein, Fredericken_ZA
dc.date.accessioned2015-11-18T03:47:25Z
dc.date.available2015-11-18T03:47:25Z
dc.date.issued2011en_ZA
dc.description.abstractBACKGROUND: Quantitative noninvasive imaging of myocardial mechanics in mice enables studies of the roles of individual genes in cardiac function. We sought to develop comprehensive three-dimensional methods for imaging myocardial mechanics in mice. METHODS: A 3D cine DENSE pulse sequence was implemented on a 7T small-bore scanner. The sequence used three-point phase cycling for artifact suppression and a stack-of-spirals k-space trajectory for efficient data acquisition. A semi-automatic 2D method was adapted for 3D image segmentation, and automated 3D methods to calculate strain, twist, and torsion were employed. A scan protocol that covered the majority of the left ventricle in a scan time of less than 25 minutes was developed, and seven healthy C57Bl/6 mice were studied. RESULTS: Using these methods, multiphase normal and shear strains were measured, as were myocardial twist and torsion. Peak end-systolic values for the normal strains at the mid-ventricular level were 0.29 +/- 0.17, -0.13 +/- 0.03, and -0.18 +/- 0.14 for Err, Ecc, and Ell, respectively. Peak end-systolic values for the shear strains were 0.00 +/- 0.08, 0.04 +/- 0.12, and 0.03 +/- 0.07 for Erc, Erl, and Ecl, respectively. The peak end-systolic normalized torsion was 5.6 +/- 0.9degrees. CONCLUSIONS: Using a 3D cine DENSE sequence tailored for cardiac imaging in mice at 7 T, a comprehensive assessment of 3D myocardial mechanics can be achieved with a scan time of less than 25 minutes and an image analysis time of approximately 1 hour.en_ZA
dc.identifier.apacitationZhong, X., Gibberman, L., Spottiswoode, B., Gilliam, A., Meyer, C., French, B., & Epstein, F. (2011). Comprehensive Cardiovascular magnetic resonance of myocardial mechanics in mice using three-dimensional cine DENSE. <i>Journal of Cardiovascular Magnetic Resonance</i>, http://hdl.handle.net/11427/15050en_ZA
dc.identifier.chicagocitationZhong, Xiaodong, Lauren Gibberman, Bruce Spottiswoode, Andrew Gilliam, Craig Meyer, Brent French, and Frederick Epstein "Comprehensive Cardiovascular magnetic resonance of myocardial mechanics in mice using three-dimensional cine DENSE." <i>Journal of Cardiovascular Magnetic Resonance</i> (2011) http://hdl.handle.net/11427/15050en_ZA
dc.identifier.citationZhong, X., Gibberman, L. B., Spottiswoode, B. S., Gilliam, A. D., Meyer, C. H., French, B. A., & Epstein, F. H. (2011). Comprehensive cardiovascular magnetic resonance of myocardial mechanics in mice using three-dimensional cine DENSE. J Cardiovasc Magn Reson, 13, 83.en_ZA
dc.identifier.ris TY - Journal Article AU - Zhong, Xiaodong AU - Gibberman, Lauren AU - Spottiswoode, Bruce AU - Gilliam, Andrew AU - Meyer, Craig AU - French, Brent AU - Epstein, Frederick AB - BACKGROUND: Quantitative noninvasive imaging of myocardial mechanics in mice enables studies of the roles of individual genes in cardiac function. We sought to develop comprehensive three-dimensional methods for imaging myocardial mechanics in mice. METHODS: A 3D cine DENSE pulse sequence was implemented on a 7T small-bore scanner. The sequence used three-point phase cycling for artifact suppression and a stack-of-spirals k-space trajectory for efficient data acquisition. A semi-automatic 2D method was adapted for 3D image segmentation, and automated 3D methods to calculate strain, twist, and torsion were employed. A scan protocol that covered the majority of the left ventricle in a scan time of less than 25 minutes was developed, and seven healthy C57Bl/6 mice were studied. RESULTS: Using these methods, multiphase normal and shear strains were measured, as were myocardial twist and torsion. Peak end-systolic values for the normal strains at the mid-ventricular level were 0.29 +/- 0.17, -0.13 +/- 0.03, and -0.18 +/- 0.14 for Err, Ecc, and Ell, respectively. Peak end-systolic values for the shear strains were 0.00 +/- 0.08, 0.04 +/- 0.12, and 0.03 +/- 0.07 for Erc, Erl, and Ecl, respectively. The peak end-systolic normalized torsion was 5.6 +/- 0.9degrees. CONCLUSIONS: Using a 3D cine DENSE sequence tailored for cardiac imaging in mice at 7 T, a comprehensive assessment of 3D myocardial mechanics can be achieved with a scan time of less than 25 minutes and an image analysis time of approximately 1 hour. DA - 2011 DB - OpenUCT DO - 10.1186/1532-429X-13-83 DP - University of Cape Town J1 - Journal of Cardiovascular Magnetic Resonance LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Comprehensive Cardiovascular magnetic resonance of myocardial mechanics in mice using three-dimensional cine DENSE TI - Comprehensive Cardiovascular magnetic resonance of myocardial mechanics in mice using three-dimensional cine DENSE UR - http://hdl.handle.net/11427/15050 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15050
dc.identifier.urihttp://dx.doi.org/10.1186/1532-429X-13-83
dc.identifier.vancouvercitationZhong X, Gibberman L, Spottiswoode B, Gilliam A, Meyer C, French B, et al. Comprehensive Cardiovascular magnetic resonance of myocardial mechanics in mice using three-dimensional cine DENSE. Journal of Cardiovascular Magnetic Resonance. 2011; http://hdl.handle.net/11427/15050.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentMRC/UCT Medical Imaging Research Uniten_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2011 Zhong et al; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceJournal of Cardiovascular Magnetic Resonanceen_ZA
dc.source.urihttp://www.jcmr-online.com/en_ZA
dc.subject.otherNoninvasive imagingen_ZA
dc.subject.other3D cine DENSE pulse sequenceen_ZA
dc.titleComprehensive Cardiovascular magnetic resonance of myocardial mechanics in mice using three-dimensional cine DENSEen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Zhong_Cardiovascular_MRI_2011.pdf
Size:
5.34 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles