Characterisation of STEC and other diarrheic E. coli isolated on CHROMagar™STEC at a tertiary referral hospital, Cape Town
| dc.contributor.author | Kalule, John B | |
| dc.contributor.author | Keddy, Karen H | |
| dc.contributor.author | Nicol, Mark P | |
| dc.date.accessioned | 2018-07-06T10:46:33Z | |
| dc.date.available | 2018-07-06T10:46:33Z | |
| dc.date.issued | 2018-06-08 | |
| dc.date.updated | 2018-06-10T03:38:24Z | |
| dc.description.abstract | Abstract Background Shiga toxin producing E. coli (STEC) is an emerging zoonotic pathogen that can cause acute renal failure, especially in children. Clinical microbiology laboratories may fail to detect STEC and other diarrhoeic E. coli unless purposive rigorous screening procedures are followed using appropriate diagnostic technology; CHROMagar™STEC has rarely been used for isolation of African diarrhoeic E. coli hence characteristics of isolates on this medium are not yet fully understood. This study aimed to determine the prevalence and characteristics of STEC and other diarrhoeic E. coli isolated on CHROMagar™STEC from stool samples submitted to the microbiology laboratory of a South African public sector tertiary care hospital. Results In total, 733 stool samples were tested. Of these, 4.5% (33/733) possessed diarrhoeic E. coli. Of the diarrheic E. coli, 5/33 (15.2%) were STEC, 15/33 (45.5%) EAggEC, 6/33 (18.2%) atypical EPEC, 5/33 (15.2%) typical EPEC, and 1/33 (3%) DAEC. None of the STEC isolates had been identified by routine testing (based on using sorbitol media to test for E. coli O157: H7 strains and not the other STEC) in the laboratory. Of the 33 strains, 55% (95% CI = 40.8–72.7) showed resistance to ampicillin. Conclusions CHROMagar™STEC enabled detection of tellurite - resistant diarrhoeic E. coli that would be missed using routine methods. Further studies are needed to determine the proportion and characteristics of those which might have been missed using this approach. | |
| dc.identifier.apacitation | Kalule, J. B., Keddy, K. H., & Nicol, M. P. (2018). Characterisation of STEC and other diarrheic E. coli isolated on CHROMagar™STEC at a tertiary referral hospital, Cape Town. <i>BMC Microbiology</i>, http://hdl.handle.net/11427/28281 | en_ZA |
| dc.identifier.chicagocitation | Kalule, John B, Karen H Keddy, and Mark P Nicol "Characterisation of STEC and other diarrheic E. coli isolated on CHROMagar™STEC at a tertiary referral hospital, Cape Town." <i>BMC Microbiology</i> (2018) http://hdl.handle.net/11427/28281 | en_ZA |
| dc.identifier.citation | Kalule, J. B., Keddy, K. H., & Nicol, M. P. (2018). Characterisation of STEC and other diarrheic E. coli isolated on CHROMagar™ STEC at a tertiary referral hospital, Cape Town. BMC microbiology, 18(1), 55. | |
| dc.identifier.ris | TY - Journal Article AU - Kalule, John B AU - Keddy, Karen H AU - Nicol, Mark P AB - Abstract Background Shiga toxin producing E. coli (STEC) is an emerging zoonotic pathogen that can cause acute renal failure, especially in children. Clinical microbiology laboratories may fail to detect STEC and other diarrhoeic E. coli unless purposive rigorous screening procedures are followed using appropriate diagnostic technology; CHROMagar™STEC has rarely been used for isolation of African diarrhoeic E. coli hence characteristics of isolates on this medium are not yet fully understood. This study aimed to determine the prevalence and characteristics of STEC and other diarrhoeic E. coli isolated on CHROMagar™STEC from stool samples submitted to the microbiology laboratory of a South African public sector tertiary care hospital. Results In total, 733 stool samples were tested. Of these, 4.5% (33/733) possessed diarrhoeic E. coli. Of the diarrheic E. coli, 5/33 (15.2%) were STEC, 15/33 (45.5%) EAggEC, 6/33 (18.2%) atypical EPEC, 5/33 (15.2%) typical EPEC, and 1/33 (3%) DAEC. None of the STEC isolates had been identified by routine testing (based on using sorbitol media to test for E. coli O157: H7 strains and not the other STEC) in the laboratory. Of the 33 strains, 55% (95% CI = 40.8–72.7) showed resistance to ampicillin. Conclusions CHROMagar™STEC enabled detection of tellurite - resistant diarrhoeic E. coli that would be missed using routine methods. Further studies are needed to determine the proportion and characteristics of those which might have been missed using this approach. DA - 2018-06-08 DB - OpenUCT DP - University of Cape Town J1 - BMC Microbiology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2018 T1 - Characterisation of STEC and other diarrheic E. coli isolated on CHROMagar™STEC at a tertiary referral hospital, Cape Town TI - Characterisation of STEC and other diarrheic E. coli isolated on CHROMagar™STEC at a tertiary referral hospital, Cape Town UR - http://hdl.handle.net/11427/28281 ER - | en_ZA |
| dc.identifier.uri | https://doi.org/10.1186/s12866-018-1195-7 | |
| dc.identifier.uri | http://hdl.handle.net/11427/28281 | |
| dc.identifier.vancouvercitation | Kalule JB, Keddy KH, Nicol MP. Characterisation of STEC and other diarrheic E. coli isolated on CHROMagar™STEC at a tertiary referral hospital, Cape Town. BMC Microbiology. 2018; http://hdl.handle.net/11427/28281. | en_ZA |
| dc.language.iso | en | |
| dc.publisher | BioMed Central | |
| dc.publisher.department | Division of Medical Microbiology | en_ZA |
| dc.publisher.faculty | Faculty of Health Sciences | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.rights.holder | The Author(s). | |
| dc.source | BMC Microbiology | |
| dc.source.uri | https://bmcmicrobiol.biomedcentral.com/ | |
| dc.subject.other | CHROMagar™STEC | |
| dc.subject.other | Tellurite resistant diarrhoeic E. coli | |
| dc.subject.other | STEC | |
| dc.subject.other | Shiga toxin | |
| dc.subject.other | Africa | |
| dc.title | Characterisation of STEC and other diarrheic E. coli isolated on CHROMagar™STEC at a tertiary referral hospital, Cape Town | |
| dc.type | Journal Article | |
| uct.type.filetype | ||
| uct.type.filetype | Text | |
| uct.type.filetype | Image |