Arteannuin-B and (3-Chlorophenyl)-2-Spiroisoxazoline Derivative Exhibit Anti-Inflammatory Effects in LPS-Activated RAW 264.7 Macrophages and BALB/c Mice-Induced Proinflammatory Responses via Downregulation of NF-κB/P38 MAPK Signaling
| dc.contributor.author | Sawhney, Gifty | |
| dc.contributor.author | Rasool, Javeed Ur | |
| dc.contributor.author | Saroch, Diksha | |
| dc.contributor.author | Ozturk, Mumin | |
| dc.contributor.author | Brombacher, Frank | |
| dc.contributor.author | Ahmad, Bilal | |
| dc.contributor.author | Bhagat, Asha | |
| dc.contributor.author | Ali, Asif | |
| dc.contributor.author | Parihar, Suraj P. | |
| dc.contributor.author | Ahmed, Zabeer | |
| dc.date.accessioned | 2024-04-29T10:29:59Z | |
| dc.date.available | 2024-04-29T10:29:59Z | |
| dc.date.issued | 2022-11-20 | |
| dc.date.updated | 2022-11-24T14:43:28Z | |
| dc.description.abstract | Host inflammatory responses are key to protection against injury; however, persistent inflammation is detrimental and contributes to morbidity and mortality. Herein, we demonstrated the anti-inflammatory role of Arteannuin-B (<b>1</b>) and its new spirocyclic-2-isoxazoline derivative <b>JR-9</b> and their side effects in acute inflammatory condition in vivo using LPS-induced cytokines assay, carrageenan-induced paw edema, acetic acid-induced writhing and tail immersion. The results show that the spirocyclic-2-isoxazoline derivative is a potent anti-inflammatory agent with minimal cell toxicity as compared to Arteannuin-B. In addition, the efficacies of these compounds were also validated by flow cytometric, computational, and histopathological analysis. Our results show that the anti-inflammatory response of <b>JR-9</b> significantly reduces the ability of mouse macrophages to produce NO, TNF-α, and IL-6 following LPS stimulation. Therefore, JR-9 is a prospective candidate for the development of anti-inflammatory drugs and its molecular mechanism is likely related to the regulation of NF-κB and MAPK signaling pathway. | |
| dc.identifier | doi: 10.3390/molecules27228068 | |
| dc.identifier.apacitation | Sawhney, G., Rasool, J. U., Saroch, D., Ozturk, M., Brombacher, F., Ahmad, B., ... Ahmed, Z. (2022). Arteannuin-B and (3-Chlorophenyl)-2-Spiroisoxazoline Derivative Exhibit Anti-Inflammatory Effects in LPS-Activated RAW 264.7 Macrophages and BALB/c Mice-Induced Proinflammatory Responses via Downregulation of NF-κB/P38 MAPK Signaling. http://hdl.handle.net/11427/39483 | en_ZA |
| dc.identifier.chicagocitation | Sawhney, Gifty, Javeed Ur Rasool, Diksha Saroch, Mumin Ozturk, Frank Brombacher, Bilal Ahmad, Asha Bhagat, Asif Ali, Suraj P. Parihar, and Zabeer Ahmed "Arteannuin-B and (3-Chlorophenyl)-2-Spiroisoxazoline Derivative Exhibit Anti-Inflammatory Effects in LPS-Activated RAW 264.7 Macrophages and BALB/c Mice-Induced Proinflammatory Responses via Downregulation of NF-κB/P38 MAPK Signaling." (2022) http://hdl.handle.net/11427/39483 | en_ZA |
| dc.identifier.citation | Molecules 27 (22): 8068 (2022) | |
| dc.identifier.ris | TY - Journal Article AU - Sawhney, Gifty AU - Rasool, Javeed Ur AU - Saroch, Diksha AU - Ozturk, Mumin AU - Brombacher, Frank AU - Ahmad, Bilal AU - Bhagat, Asha AU - Ali, Asif AU - Parihar, Suraj P. AU - Ahmed, Zabeer AB - Host inflammatory responses are key to protection against injury; however, persistent inflammation is detrimental and contributes to morbidity and mortality. Herein, we demonstrated the anti-inflammatory role of Arteannuin-B (<b>1</b>) and its new spirocyclic-2-isoxazoline derivative <b>JR-9</b> and their side effects in acute inflammatory condition in vivo using LPS-induced cytokines assay, carrageenan-induced paw edema, acetic acid-induced writhing and tail immersion. The results show that the spirocyclic-2-isoxazoline derivative is a potent anti-inflammatory agent with minimal cell toxicity as compared to Arteannuin-B. In addition, the efficacies of these compounds were also validated by flow cytometric, computational, and histopathological analysis. Our results show that the anti-inflammatory response of <b>JR-9</b> significantly reduces the ability of mouse macrophages to produce NO, TNF-α, and IL-6 following LPS stimulation. Therefore, JR-9 is a prospective candidate for the development of anti-inflammatory drugs and its molecular mechanism is likely related to the regulation of NF-κB and MAPK signaling pathway. DA - 2022-11-20 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PY - 2022 T1 - Arteannuin-B and (3-Chlorophenyl)-2-Spiroisoxazoline Derivative Exhibit Anti-Inflammatory Effects in LPS-Activated RAW 264.7 Macrophages and BALB/c Mice-Induced Proinflammatory Responses via Downregulation of NF-κB/P38 MAPK Signaling TI - Arteannuin-B and (3-Chlorophenyl)-2-Spiroisoxazoline Derivative Exhibit Anti-Inflammatory Effects in LPS-Activated RAW 264.7 Macrophages and BALB/c Mice-Induced Proinflammatory Responses via Downregulation of NF-κB/P38 MAPK Signaling UR - http://hdl.handle.net/11427/39483 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/39483 | |
| dc.identifier.vancouvercitation | Sawhney G, Rasool JU, Saroch D, Ozturk M, Brombacher F, Ahmad B, et al. Arteannuin-B and (3-Chlorophenyl)-2-Spiroisoxazoline Derivative Exhibit Anti-Inflammatory Effects in LPS-Activated RAW 264.7 Macrophages and BALB/c Mice-Induced Proinflammatory Responses via Downregulation of NF-κB/P38 MAPK Signaling. 2022; http://hdl.handle.net/11427/39483. | en_ZA |
| dc.title | Arteannuin-B and (3-Chlorophenyl)-2-Spiroisoxazoline Derivative Exhibit Anti-Inflammatory Effects in LPS-Activated RAW 264.7 Macrophages and BALB/c Mice-Induced Proinflammatory Responses via Downregulation of NF-κB/P38 MAPK Signaling | |
| dc.type | Journal Article |