Molecular Characterization of trans -Golgi p230: a human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil α-helical domains and a Granin Motif

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dc.contributor.authorErlich, Rebecca
dc.contributor.authorGleeson, Paul A
dc.contributor.authorCampbell, Paul
dc.contributor.authorDietzsch, Erin
dc.contributor.authorToh, Ban-Hock
dc.date.accessioned2021-10-08T07:20:45Z
dc.date.available2021-10-08T07:20:45Z
dc.date.issued1996
dc.description.abstractUsing autoantibodies from a Sjögren's syndrome patient, we have previously identified a 230-kDa peripheral membrane protein associated with the cytosolic face of the trans-Golgi (Kooy, J., Toh, B. H., Pettitt, J. M., Erlich, R. and Gleeson, P. A. (1992) J. Biol. Chem. 267, 20255-20263). Here we report the molecular cloning and sequence analysis of human p230 and the localization of its gene to chromosome 6p12 22. Partial cDNA clones, isolated from a HeLa cell cDNA library using autoantibodies, were used to obtain additional cDNAs, which together span 7695 base pairs (bp). The p230 mRNA is approximately 7.7 kilobases. Two alternatively spliced mRNAs for p230 were detected. These differed by 21- and 63-bp insertions in the 3'-sequence, resulting in differences in amino acid sequence at the carboxyl terminus. The predicted 261-kDa protein is highly hydrophilic with 17-20% homology with many proteins containing coiled-coil domains. Apart from two proline-rich regions (amino acids 1-117 and 239-270), p230 contains a very high frequency of heptad repeats, characteristic of alpha-helices that form dimeric coiled-coil structures. p230 also includes the sequence ESLALEELEL (amino acids 538-546), a motif found in the granin family of acidic proteins present in secretory granules of neuroendocrine cells. This is the first report of a cytosolic Golgi protein containing a granin motif. The structural characteristics of p230 indicate that it may play a role in vesicular transport from the trans-Golgi.
dc.identifier.apacitationErlich, R., Gleeson, P. A., Campbell, P., Dietzsch, E., & Toh, B. (1996). Molecular Characterization of trans -Golgi p230: a human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil α-helical domains and a Granin Motif. <i>The Journal of Biological Chemistry</i>, 271(14), 8328 - 8337. http://hdl.handle.net/11427/34996en_ZA
dc.identifier.chicagocitationErlich, Rebecca, Paul A Gleeson, Paul Campbell, Erin Dietzsch, and Ban-Hock Toh "Molecular Characterization of trans -Golgi p230: a human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil α-helical domains and a Granin Motif." <i>The Journal of Biological Chemistry</i> 271, 14. (1996): 8328 - 8337. http://hdl.handle.net/11427/34996en_ZA
dc.identifier.citationErlich, R., Gleeson, P.A., Campbell, P., Dietzsch, E. & Toh, B. 1996. Molecular Characterization of trans -Golgi p230: a human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil α-helical domains and a Granin Motif. <i>The Journal of Biological Chemistry.</i> 271(14):8328 - 8337. http://hdl.handle.net/11427/34996en_ZA
dc.identifier.issn0021-9258
dc.identifier.issn1083-351X
dc.identifier.ris TY - Journal Article AU - Erlich, Rebecca AU - Gleeson, Paul A AU - Campbell, Paul AU - Dietzsch, Erin AU - Toh, Ban-Hock AB - Using autoantibodies from a Sjögren's syndrome patient, we have previously identified a 230-kDa peripheral membrane protein associated with the cytosolic face of the trans-Golgi (Kooy, J., Toh, B. H., Pettitt, J. M., Erlich, R. and Gleeson, P. A. (1992) J. Biol. Chem. 267, 20255-20263). Here we report the molecular cloning and sequence analysis of human p230 and the localization of its gene to chromosome 6p12 22. Partial cDNA clones, isolated from a HeLa cell cDNA library using autoantibodies, were used to obtain additional cDNAs, which together span 7695 base pairs (bp). The p230 mRNA is approximately 7.7 kilobases. Two alternatively spliced mRNAs for p230 were detected. These differed by 21- and 63-bp insertions in the 3'-sequence, resulting in differences in amino acid sequence at the carboxyl terminus. The predicted 261-kDa protein is highly hydrophilic with 17-20% homology with many proteins containing coiled-coil domains. Apart from two proline-rich regions (amino acids 1-117 and 239-270), p230 contains a very high frequency of heptad repeats, characteristic of alpha-helices that form dimeric coiled-coil structures. p230 also includes the sequence ESLALEELEL (amino acids 538-546), a motif found in the granin family of acidic proteins present in secretory granules of neuroendocrine cells. This is the first report of a cytosolic Golgi protein containing a granin motif. The structural characteristics of p230 indicate that it may play a role in vesicular transport from the trans-Golgi. DA - 1996 DB - OpenUCT DP - University of Cape Town IS - 14 J1 - The Journal of Biological Chemistry LK - https://open.uct.ac.za PY - 1996 SM - 0021-9258 SM - 1083-351X T1 - Molecular Characterization of trans -Golgi p230: a human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil α-helical domains and a Granin Motif TI - Molecular Characterization of trans -Golgi p230: a human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil α-helical domains and a Granin Motif UR - http://hdl.handle.net/11427/34996 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/34996
dc.identifier.vancouvercitationErlich R, Gleeson PA, Campbell P, Dietzsch E, Toh B. Molecular Characterization of trans -Golgi p230: a human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil α-helical domains and a Granin Motif. The Journal of Biological Chemistry. 1996;271(14):8328 - 8337. http://hdl.handle.net/11427/34996.en_ZA
dc.language.isoeng
dc.publisher.departmentDivision of Biomedical Engineering
dc.publisher.facultyFaculty of Health Sciences
dc.sourceThe Journal of Biological Chemistry
dc.source.journalissue14
dc.source.journalvolume271
dc.source.pagination8328 - 8337
dc.source.urihttps://dx.doi.org/10.1074/jbc.271.14.8328
dc.subject.otherAmino Acid Sequence
dc.subject.otherAutoantigens
dc.subject.otherBase Sequence
dc.subject.otherCell Compartmentation
dc.subject.otherChromosomes, Human, Pair 6
dc.subject.otherCloning, Molecular
dc.subject.otherDNA, Complementary
dc.subject.otherFluorescent Antibody Technique, Indirect
dc.subject.otherGene Expression
dc.subject.otherGolgi Apparatus
dc.subject.otherHumans
dc.subject.otherIntracellular Membranes
dc.subject.otherMembrane Proteins
dc.subject.otherMolecular Sequence Data
dc.subject.otherProtein Structure, Secondary
dc.subject.otherRNA, Messenger
dc.titleMolecular Characterization of trans -Golgi p230: a human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil α-helical domains and a Granin Motif
dc.typeJournal Article
uct.type.publicationResearch
uct.type.resourceJournal Article
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