Characterisation of the Complex Microbial Community Associated with the ASTER™ Thiocyanate Biodegradation System

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dc.contributor.authorHuddy, Robert J
dc.contributor.authorVan Zyl, A Wynand
dc.contributor.authorVan Hille, Robert P
dc.contributor.authorHarrison, Susan TL
dc.date.accessioned2018-03-01T11:15:35Z
dc.date.available2018-03-01T11:15:35Z
dc.date.issued2015-05-15
dc.description.abstractThe ASTER™ process is used to bioremediate cyanide- (CN-) and thiocyanate- (SCN-) 13 containing waste water. This aerobic process is able to reduce the CN- and SCN14 concentrations to below 1 mg/L efficiently in a continuous system, facilitating reuse of 15 process water or safe discharge. Such remediation systems, which completely eliminate risk 16 associated with the pollutants, are essential for sustainable mineral processing and the long 17 term minimisation of environmental burden through both pollutant destruction and exploiting 18 opportunities for nutrient recycle. Process robustness of these bioremediation options can be 19 enhanced by good understanding of the microbial community involved in the process. To 20 date, the microbial consortia associated with the ASTER™ bioprocess have been poorly 21 characterised using isolation approaches only. As a result, the relative abundance and 22 diversity of the community has been significantly under-represented. In this study, both planktonic and biofilm-associated biomass have been observed. 23 Microscopy has revealed the 24 diversity of these communities, including bacteria, motile eukaryotes, filamentous fungi and 25 algae, with the biofilm densely packed with microorganisms. The results of the molecular 26 characterisation study reported here, using a clone library approach, demonstrate that the 27 microbial community associated with the ASTER™ bioprocess system is far more complex 28 than previously suggested, with over 30 bacterial species identified thus far. On-going 29 investigations focus on identification of key microbial community members associated with 30 SCN- biodegradation and other critical metabolic functions, as well as the expected dynamic 31 response of this complex microbial community to shifts in the operating window of the 32 process.en_ZA
dc.identifier.apacitationHuddy, R. J., Van Zyl, A. W., Van Hille, R. P., & Harrison, S. T. (2015). Characterisation of the Complex Microbial Community Associated with the ASTER™ Thiocyanate Biodegradation System. <i>Minerals Engineering</i>, http://hdl.handle.net/11427/27613en_ZA
dc.identifier.chicagocitationHuddy, Robert J, A Wynand Van Zyl, Robert P Van Hille, and Susan TL Harrison "Characterisation of the Complex Microbial Community Associated with the ASTER™ Thiocyanate Biodegradation System." <i>Minerals Engineering</i> (2015) http://hdl.handle.net/11427/27613en_ZA
dc.identifier.citationHuddy RJ, van Zyl AW, van Hille RP, Harrison STL, Characterisation of the complex microbial community associated with the ASTER™ thiocyanate biodegradation system, Minerals Engineering, Volume 76, 15 May 2015, Pages 65-71en_ZA
dc.identifier.ris TY - Journal Article AU - Huddy, Robert J AU - Van Zyl, A Wynand AU - Van Hille, Robert P AU - Harrison, Susan TL AB - The ASTER™ process is used to bioremediate cyanide- (CN-) and thiocyanate- (SCN-) 13 containing waste water. This aerobic process is able to reduce the CN- and SCN14 concentrations to below 1 mg/L efficiently in a continuous system, facilitating reuse of 15 process water or safe discharge. Such remediation systems, which completely eliminate risk 16 associated with the pollutants, are essential for sustainable mineral processing and the long 17 term minimisation of environmental burden through both pollutant destruction and exploiting 18 opportunities for nutrient recycle. Process robustness of these bioremediation options can be 19 enhanced by good understanding of the microbial community involved in the process. To 20 date, the microbial consortia associated with the ASTER™ bioprocess have been poorly 21 characterised using isolation approaches only. As a result, the relative abundance and 22 diversity of the community has been significantly under-represented. In this study, both planktonic and biofilm-associated biomass have been observed. 23 Microscopy has revealed the 24 diversity of these communities, including bacteria, motile eukaryotes, filamentous fungi and 25 algae, with the biofilm densely packed with microorganisms. The results of the molecular 26 characterisation study reported here, using a clone library approach, demonstrate that the 27 microbial community associated with the ASTER™ bioprocess system is far more complex 28 than previously suggested, with over 30 bacterial species identified thus far. On-going 29 investigations focus on identification of key microbial community members associated with 30 SCN- biodegradation and other critical metabolic functions, as well as the expected dynamic 31 response of this complex microbial community to shifts in the operating window of the 32 process. DA - 2015-05-15 DB - OpenUCT DP - University of Cape Town J1 - Minerals Engineering LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - Characterisation of the Complex Microbial Community Associated with the ASTER™ Thiocyanate Biodegradation System TI - Characterisation of the Complex Microbial Community Associated with the ASTER™ Thiocyanate Biodegradation System UR - http://hdl.handle.net/11427/27613 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/27613
dc.identifier.vancouvercitationHuddy RJ, Van Zyl AW, Van Hille RP, Harrison ST. Characterisation of the Complex Microbial Community Associated with the ASTER™ Thiocyanate Biodegradation System. Minerals Engineering. 2015; http://hdl.handle.net/11427/27613.en_ZA
dc.languageengen_ZA
dc.publisherElsevieren_ZA
dc.publisher.departmentCentre for Bioprocess Engineering Research
dc.publisher.facultyFaculty of Engineering and the Built Environment
dc.publisher.institutionUniversity of Cape Town
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_ZA
dc.sourceMinerals Engineeringen_ZA
dc.source.urihttps://www.journals.elsevier.com/minerals-engineering/
dc.titleCharacterisation of the Complex Microbial Community Associated with the ASTER™ Thiocyanate Biodegradation Systemen_ZA
dc.typeJournal Articleen_ZA
uct.subject.keywordsGold oresen_ZA
uct.subject.keywordsCyanidationen_ZA
uct.subject.keywordsThiocyanateen_ZA
uct.subject.keywordsBiological treatmenten_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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