Pre-Clinical Evaluation of Tenofovir and Tenofovir Alafenamide for HIV-1 Pre-Exposure Prophylaxis in Foreskin Tissue
| dc.contributor.author | Else, Laura | |
| dc.contributor.author | Penchala, Sujan D | |
| dc.contributor.author | Pillay, Azure-Dee | |
| dc.contributor.author | Seiphetlo, Thabiso B | |
| dc.contributor.author | Lebina, Limakatso | |
| dc.contributor.author | Callebaut, Christian | |
| dc.contributor.author | Minhas, Suks | |
| dc.contributor.author | Morley, Roland | |
| dc.contributor.author | Rashid, Tina | |
| dc.contributor.author | Martinson, Neil | |
| dc.contributor.author | Fox, Julie | |
| dc.contributor.author | Khoo, Saye | |
| dc.contributor.author | Herrera, Carolina | |
| dc.date.accessioned | 2022-09-14T16:04:18Z | |
| dc.date.available | 2022-09-14T16:04:18Z | |
| dc.date.issued | 2022-06-16 | |
| dc.date.updated | 2022-06-23T12:11:37Z | |
| dc.description.abstract | Background: HIV-1 pre-exposure prophylaxis (PrEP) has focused predominantly on protective efficacy in receptive sex, with limited research on the dosing requirements for insertive sex. We pre-clinically assessed the ex vivo pharmacokinetic–pharmacodynamic (PK–PD) profile of tenofovir (TFV) and tenofovir alafenamide (TAF) in foreskin tissue. Methods: Inner and outer foreskin explants were exposed to serial dilutions of TFV or TAF prior to addition of HIV-1<sub>BaL</sub> at a high (HVT) or a low viral titer (LVT). Infection was assessed by measurement of p24 in foreskin culture supernatants. TFV, TAF and TFV–diphosphate (TFV–DP) concentrations were measured in tissues, culture supernatants and dosing and washing solutions. Results: Dose–response curves were obtained for both drugs, with greater potency observed against LVT. Inhibitory equivalency mimicking oral dosing was defined between 1 mg/mL of TFV and 15 µg/mL of TAF against HVT challenge. Concentrations of TFV–DP in foreskin explants were approximately six-fold higher after ex vivo dosing with TAF than with TFV. Statistically significant negative linear correlations were observed between explant levels of TFV or TFV–DP and p24 concentrations following HVT. Conclusions: Pre-clinical evaluation of TAF in foreskin explants revealed greater potency than TFV against penile HIV transmission. Clinical evaluation is underway to support this finding. | en_US |
| dc.identifier | doi: 10.3390/pharmaceutics14061285 | |
| dc.identifier.apacitation | Else, L., Penchala, S. D., Pillay, A., Seiphetlo, T. B., Lebina, L., Callebaut, C., ... Herrera, C. (2022). Pre-Clinical Evaluation of Tenofovir and Tenofovir Alafenamide for HIV-1 Pre-Exposure Prophylaxis in Foreskin Tissue. <i>Pharmaceutics</i>, 14(6), 1285. http://hdl.handle.net/11427/36810 | en_ZA |
| dc.identifier.chicagocitation | Else, Laura, Sujan D Penchala, Azure-Dee Pillay, Thabiso B Seiphetlo, Limakatso Lebina, Christian Callebaut, Suks Minhas, et al "Pre-Clinical Evaluation of Tenofovir and Tenofovir Alafenamide for HIV-1 Pre-Exposure Prophylaxis in Foreskin Tissue." <i>Pharmaceutics</i> 14, 6. (2022): 1285. http://hdl.handle.net/11427/36810 | en_ZA |
| dc.identifier.citation | Else, L., Penchala, S.D., Pillay, A., Seiphetlo, T.B., Lebina, L., Callebaut, C., Minhas, S. & Morley, R. et al. 2022. Pre-Clinical Evaluation of Tenofovir and Tenofovir Alafenamide for HIV-1 Pre-Exposure Prophylaxis in Foreskin Tissue. <i>Pharmaceutics.</i> 14(6):1285. http://hdl.handle.net/11427/36810 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Else, Laura AU - Penchala, Sujan D AU - Pillay, Azure-Dee AU - Seiphetlo, Thabiso B AU - Lebina, Limakatso AU - Callebaut, Christian AU - Minhas, Suks AU - Morley, Roland AU - Rashid, Tina AU - Martinson, Neil AU - Fox, Julie AU - Khoo, Saye AU - Herrera, Carolina AB - Background: HIV-1 pre-exposure prophylaxis (PrEP) has focused predominantly on protective efficacy in receptive sex, with limited research on the dosing requirements for insertive sex. We pre-clinically assessed the ex vivo pharmacokinetic–pharmacodynamic (PK–PD) profile of tenofovir (TFV) and tenofovir alafenamide (TAF) in foreskin tissue. Methods: Inner and outer foreskin explants were exposed to serial dilutions of TFV or TAF prior to addition of HIV-1<sub>BaL</sub> at a high (HVT) or a low viral titer (LVT). Infection was assessed by measurement of p24 in foreskin culture supernatants. TFV, TAF and TFV–diphosphate (TFV–DP) concentrations were measured in tissues, culture supernatants and dosing and washing solutions. Results: Dose–response curves were obtained for both drugs, with greater potency observed against LVT. Inhibitory equivalency mimicking oral dosing was defined between 1 mg/mL of TFV and 15 µg/mL of TAF against HVT challenge. Concentrations of TFV–DP in foreskin explants were approximately six-fold higher after ex vivo dosing with TAF than with TFV. Statistically significant negative linear correlations were observed between explant levels of TFV or TFV–DP and p24 concentrations following HVT. Conclusions: Pre-clinical evaluation of TAF in foreskin explants revealed greater potency than TFV against penile HIV transmission. Clinical evaluation is underway to support this finding. DA - 2022-06-16 DB - OpenUCT DP - University of Cape Town IS - 6 J1 - Pharmaceutics LK - https://open.uct.ac.za PY - 2022 T1 - Pre-Clinical Evaluation of Tenofovir and Tenofovir Alafenamide for HIV-1 Pre-Exposure Prophylaxis in Foreskin Tissue TI - Pre-Clinical Evaluation of Tenofovir and Tenofovir Alafenamide for HIV-1 Pre-Exposure Prophylaxis in Foreskin Tissue UR - http://hdl.handle.net/11427/36810 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/36810 | |
| dc.identifier.vancouvercitation | Else L, Penchala SD, Pillay A, Seiphetlo TB, Lebina L, Callebaut C, et al. Pre-Clinical Evaluation of Tenofovir and Tenofovir Alafenamide for HIV-1 Pre-Exposure Prophylaxis in Foreskin Tissue. Pharmaceutics. 2022;14(6):1285. http://hdl.handle.net/11427/36810. | en_ZA |
| dc.language.iso | en | en_US |
| dc.publisher.department | Division of Immunology | en_US |
| dc.publisher.faculty | Faculty of Health Sciences | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
| dc.source | Pharmaceutics | en_US |
| dc.source.journalissue | 6 | en_US |
| dc.source.journalvolume | 14 | en_US |
| dc.source.pagination | 1285 | en_US |
| dc.source.uri | https://www.mdpi.com/journal/pharmaceutics | |
| dc.title | Pre-Clinical Evaluation of Tenofovir and Tenofovir Alafenamide for HIV-1 Pre-Exposure Prophylaxis in Foreskin Tissue | en_US |
| dc.type | Journal Article | en_US |