Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts

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dc.contributor.authorHabte, Habtomen_ZA
dc.contributor.authorde Beer, Corenaen_ZA
dc.contributor.authorLotz, Zoeen_ZA
dc.contributor.authorRoux, Paulen_ZA
dc.contributor.authorMall, Anwaren_ZA
dc.date.accessioned2015-11-11T11:56:13Z
dc.date.available2015-11-11T11:56:13Z
dc.date.issued2010en_ZA
dc.description.abstractBACKGROUND: We have previously shown that MUC5B and MUC7 mucins from saliva of HIV negative individuals inhibit HIV-1 activity by 100% in an in vitro assay. The purpose of this subsequent study was to investigate whether MUC5B and MUC7 from saliva of HIV patients or with full blown AIDS had a similar inhibitory activity against the virus. METHODS: Salivary MUC5B and MUC7 from HIV patients with different CD4 counts (< 200, 200-400 and > 400) were incubated with HIV-1 prior to infection of the human T lymphoblastoid cell line (CEM SS cells). Cells were then cultured and viral replication was measured by a qualitative p24 antigen assay. The size, charge and immunoreactivity of mucins from HIV negative and positive individuals was also analysed by SDS-PAGE, Western blot and ELISA respectively. RESULTS: It was shown that irrespective of their CD4 counts both MUC5B and MUC7 from HIV patients, unlike the MUC5B and MUC7 from HIV negative individuals, did not inhibit HIV-1 activity. Size, charge and immunoreactivity differences between the mucins from HIV negative and positive individuals and among the mucins from HIV patients of different CD4 count was observed by SDS-PAGE, Western blot and ELISA. CONCLUSIONS: Purified salivary mucins from HIV positive patients do not inhibit the AIDS virus in an in vitro assay. Although the reason for the inability of mucins from infected individuals to inhibit the virus is not known, it is likely that there is an alteration of the glycosylation pattern, and therefore of charge of mucin, in HIV positive patients. The ability to inhibit the virus by aggregation by sugar chains is thus diminished.en_ZA
dc.identifier.apacitationHabte, H., de Beer, C., Lotz, Z., Roux, P., & Mall, A. (2010). Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts. <i>Virology Journal</i>, http://hdl.handle.net/11427/14865en_ZA
dc.identifier.chicagocitationHabte, Habtom, Corena de Beer, Zoe Lotz, Paul Roux, and Anwar Mall "Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts." <i>Virology Journal</i> (2010) http://hdl.handle.net/11427/14865en_ZA
dc.identifier.citationHabte, H. H., De Beer, C., Lotz, Z. E., Roux, P., & Mall, A. S. (2010). Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts. Virol J, 7, 269.en_ZA
dc.identifier.ris TY - Journal Article AU - Habte, Habtom AU - de Beer, Corena AU - Lotz, Zoe AU - Roux, Paul AU - Mall, Anwar AB - BACKGROUND: We have previously shown that MUC5B and MUC7 mucins from saliva of HIV negative individuals inhibit HIV-1 activity by 100% in an in vitro assay. The purpose of this subsequent study was to investigate whether MUC5B and MUC7 from saliva of HIV patients or with full blown AIDS had a similar inhibitory activity against the virus. METHODS: Salivary MUC5B and MUC7 from HIV patients with different CD4 counts (< 200, 200-400 and > 400) were incubated with HIV-1 prior to infection of the human T lymphoblastoid cell line (CEM SS cells). Cells were then cultured and viral replication was measured by a qualitative p24 antigen assay. The size, charge and immunoreactivity of mucins from HIV negative and positive individuals was also analysed by SDS-PAGE, Western blot and ELISA respectively. RESULTS: It was shown that irrespective of their CD4 counts both MUC5B and MUC7 from HIV patients, unlike the MUC5B and MUC7 from HIV negative individuals, did not inhibit HIV-1 activity. Size, charge and immunoreactivity differences between the mucins from HIV negative and positive individuals and among the mucins from HIV patients of different CD4 count was observed by SDS-PAGE, Western blot and ELISA. CONCLUSIONS: Purified salivary mucins from HIV positive patients do not inhibit the AIDS virus in an in vitro assay. Although the reason for the inability of mucins from infected individuals to inhibit the virus is not known, it is likely that there is an alteration of the glycosylation pattern, and therefore of charge of mucin, in HIV positive patients. The ability to inhibit the virus by aggregation by sugar chains is thus diminished. DA - 2010 DB - OpenUCT DO - 10.1186/1743-422X-7-269 DP - University of Cape Town J1 - Virology Journal LK - https://open.uct.ac.za PB - University of Cape Town PY - 2010 T1 - Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts TI - Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts UR - http://hdl.handle.net/11427/14865 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14865
dc.identifier.urihttp://dx.doi.org/10.1186/1743-422X-7-269
dc.identifier.vancouvercitationHabte H, de Beer C, Lotz Z, Roux P, Mall A. Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts. Virology Journal. 2010; http://hdl.handle.net/11427/14865.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDivision of General Surgeryen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2010 Habte et al; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceVirology Journalen_ZA
dc.source.urihttp://www.virologyj.com/en_ZA
dc.subject.otherHuman saliva, HIV transmission, HIV (Viruses), Oral transmission, HIV (Viruses) Researchen_ZA
dc.titleAnti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 countsen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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