Co-expression and promoter content analyses assign a role in biotic and abiotic stress responses to plant natriuretic peptides

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dc.contributor.authorMeier, Stuarten_ZA
dc.contributor.authorBastian, Reneen_ZA
dc.contributor.authorDonaldson, Laraen_ZA
dc.contributor.authorMurray, Shaneen_ZA
dc.contributor.authorBajic, Vladimiren_ZA
dc.contributor.authorGehring, Chrisen_ZA
dc.date.accessioned2015-10-28T07:03:43Z
dc.date.available2015-10-28T07:03:43Z
dc.date.issued2008en_ZA
dc.description.abstractBACKGROUND: Plant natriuretic peptides (PNPs) are a class of systemically mobile molecules distantly related to expansins. While several physiological responses to PNPs have been reported, their biological role has remained elusive. Here we use a combination of expression correlation analysis, meta-analysis of gene expression profiles in response to specific stimuli and in selected mutants, and promoter content analysis to infer the biological role of the Arabidopsis thaliana PNP, AtPNP-A. RESULTS: A gene ontology analysis of AtPNP-A and the 25 most expression correlated genes revealed a significant over representation of genes annotated as part of the systemic acquired resistance (SAR) pathway. Transcription of these genes is strongly induced in response to salicylic acid (SA) and its functional synthetic analogue benzothiadiazole S-methylester (BTH), a number of biotic and abiotic stresses including many SA-mediated SAR-inducing conditions, as well as in the constitutive SAR expressing mutants cpr5 and mpk4 which have elevated SA levels. Furthermore, the expression of AtPNP-A was determined to be significantly correlated with the SAR annotated transcription factor, WRKY 70, and the promoters of AtPNP-A and the correlated genes contain an enrichment in the core WRKY binding W-box cis-elements. In constitutively expressing WRKY 70 lines the expression of AtPNP-A and the correlated genes, including the SAR marker genes, PR-2 and PR-5, were determined to be strongly induced. CONCLUSION: The co-expression analyses, both in wild type and mutants, provides compelling evidence that suggests AtPNP-A may function as a component of plant defence responses and SAR in particular. The presented evidence also suggests that the expression of AtPNP-A is controlled by WRKY transcription factors and WRKY 70 in particular. AtPNP-A shares many characteristics with PR proteins in that its transcription is strongly induced in response to pathogen challenges, it contains an N-terminal signalling peptide and is secreted into the extracellular space and along with PR-1, PR-2 and PR-5 proteins it has been isolated from the Arabidopsis apoplast. Based on these findings we suggest that AtPNP-A could be classified as a newly identified PR protein.en_ZA
dc.identifier.apacitationMeier, S., Bastian, R., Donaldson, L., Murray, S., Bajic, V., & Gehring, C. (2008). Co-expression and promoter content analyses assign a role in biotic and abiotic stress responses to plant natriuretic peptides. <i>BMC Plant Biology</i>, http://hdl.handle.net/11427/14465en_ZA
dc.identifier.chicagocitationMeier, Stuart, Rene Bastian, Lara Donaldson, Shane Murray, Vladimir Bajic, and Chris Gehring "Co-expression and promoter content analyses assign a role in biotic and abiotic stress responses to plant natriuretic peptides." <i>BMC Plant Biology</i> (2008) http://hdl.handle.net/11427/14465en_ZA
dc.identifier.citationMeier, S., Bastian, R., Donaldson, L., Murray, S., Bajic, V., & Gehring, C. (2008). Co-expression and promoter content analyses assign a role in biotic and abiotic stress responses to plant natriuretic peptides. BMC plant biology, 8(1), 24.en_ZA
dc.identifier.ris TY - Journal Article AU - Meier, Stuart AU - Bastian, Rene AU - Donaldson, Lara AU - Murray, Shane AU - Bajic, Vladimir AU - Gehring, Chris AB - BACKGROUND: Plant natriuretic peptides (PNPs) are a class of systemically mobile molecules distantly related to expansins. While several physiological responses to PNPs have been reported, their biological role has remained elusive. Here we use a combination of expression correlation analysis, meta-analysis of gene expression profiles in response to specific stimuli and in selected mutants, and promoter content analysis to infer the biological role of the Arabidopsis thaliana PNP, AtPNP-A. RESULTS: A gene ontology analysis of AtPNP-A and the 25 most expression correlated genes revealed a significant over representation of genes annotated as part of the systemic acquired resistance (SAR) pathway. Transcription of these genes is strongly induced in response to salicylic acid (SA) and its functional synthetic analogue benzothiadiazole S-methylester (BTH), a number of biotic and abiotic stresses including many SA-mediated SAR-inducing conditions, as well as in the constitutive SAR expressing mutants cpr5 and mpk4 which have elevated SA levels. Furthermore, the expression of AtPNP-A was determined to be significantly correlated with the SAR annotated transcription factor, WRKY 70, and the promoters of AtPNP-A and the correlated genes contain an enrichment in the core WRKY binding W-box cis-elements. In constitutively expressing WRKY 70 lines the expression of AtPNP-A and the correlated genes, including the SAR marker genes, PR-2 and PR-5, were determined to be strongly induced. CONCLUSION: The co-expression analyses, both in wild type and mutants, provides compelling evidence that suggests AtPNP-A may function as a component of plant defence responses and SAR in particular. The presented evidence also suggests that the expression of AtPNP-A is controlled by WRKY transcription factors and WRKY 70 in particular. AtPNP-A shares many characteristics with PR proteins in that its transcription is strongly induced in response to pathogen challenges, it contains an N-terminal signalling peptide and is secreted into the extracellular space and along with PR-1, PR-2 and PR-5 proteins it has been isolated from the Arabidopsis apoplast. Based on these findings we suggest that AtPNP-A could be classified as a newly identified PR protein. DA - 2008 DB - OpenUCT DO - 10.1186/1471-2229-8-24 DP - University of Cape Town J1 - BMC Plant Biology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - Co-expression and promoter content analyses assign a role in biotic and abiotic stress responses to plant natriuretic peptides TI - Co-expression and promoter content analyses assign a role in biotic and abiotic stress responses to plant natriuretic peptides UR - http://hdl.handle.net/11427/14465 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14465
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2229-8-24
dc.identifier.vancouvercitationMeier S, Bastian R, Donaldson L, Murray S, Bajic V, Gehring C. Co-expression and promoter content analyses assign a role in biotic and abiotic stress responses to plant natriuretic peptides. BMC Plant Biology. 2008; http://hdl.handle.net/11427/14465.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2008 Meier et al; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceBMC Plant Biologyen_ZA
dc.source.urihttp://www.biomedcentral.com/bmcplantbiol/en_ZA
dc.subject.otherArabidopsis Proteinsen_ZA
dc.subject.otherGene Expression Profilingen_ZA
dc.subject.otherGene Expression Regulation, Planten_ZA
dc.subject.otherNatriuretic Peptidesen_ZA
dc.titleCo-expression and promoter content analyses assign a role in biotic and abiotic stress responses to plant natriuretic peptidesen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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