Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements

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dc.contributor.authorvan Rooyen, Jason
dc.contributor.authorBelrhali, Hassan
dc.contributor.authorAbratt, Valarie
dc.contributor.authorSewella, Trevor B
dc.date.accessioned2016-08-31T11:53:15Z
dc.date.available2016-08-31T11:53:15Z
dc.date.issued2011
dc.date.updated2016-08-31T11:52:00Z
dc.description.abstractThis work details the intentional modifications that led to the first structure of a type III glutamine synthetase enzyme (GSIII). This approach followed the serendipitous discovery of digestion caused by an extracellular protease from a contaminating bacterium, Pseudomonas fluorescens. The protease only cleaves the GSIII protein at a single site, leaving the oligomer intact but allowing the protein to crystallize in a different space group. This transition from space group P1 to space group C2221 is accompanied by improved growth characteristics, more reproducible diffraction and enhanced mechanical stability. The crystallographic analyses presented here provide the structural basis of the altered molecular packing in the full-length and digested crystal forms and suggest modifications for future structural studies.en_ZA
dc.identifierhttp://dx.doi.org/10.1107/S1744309110053893
dc.identifier.apacitationvan Rooyen, J., Belrhali, H., Abratt, V., & Sewella, T. B. (2011). Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements. <i>Acta Crystallographica. Section F, Structural biology and crystallization communications</i>, http://hdl.handle.net/11427/21608en_ZA
dc.identifier.chicagocitationvan Rooyen, Jason, Hassan Belrhali, Valarie Abratt, and Trevor B Sewella "Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements." <i>Acta Crystallographica. Section F, Structural biology and crystallization communications</i> (2011) http://hdl.handle.net/11427/21608en_ZA
dc.identifier.citationvan Rooyen, J., Belrhali, H., Abratt, V., & Sewell, B. T. (2011). Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 67(3), 358-363.en_ZA
dc.identifier.issn1744-3091en_ZA
dc.identifier.ris TY - Journal Article AU - van Rooyen, Jason AU - Belrhali, Hassan AU - Abratt, Valarie AU - Sewella, Trevor B AB - This work details the intentional modifications that led to the first structure of a type III glutamine synthetase enzyme (GSIII). This approach followed the serendipitous discovery of digestion caused by an extracellular protease from a contaminating bacterium, Pseudomonas fluorescens. The protease only cleaves the GSIII protein at a single site, leaving the oligomer intact but allowing the protein to crystallize in a different space group. This transition from space group P1 to space group C2221 is accompanied by improved growth characteristics, more reproducible diffraction and enhanced mechanical stability. The crystallographic analyses presented here provide the structural basis of the altered molecular packing in the full-length and digested crystal forms and suggest modifications for future structural studies. DA - 2011 DB - OpenUCT DP - University of Cape Town J1 - Acta Crystallographica. Section F, Structural biology and crystallization communications LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 SM - 1744-3091 T1 - Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements TI - Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements UR - http://hdl.handle.net/11427/21608 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21608
dc.identifier.urihttp://scripts.iucr.org/cgi-bin/paper?pu5321
dc.identifier.vancouvercitationvan Rooyen J, Belrhali H, Abratt V, Sewella TB. Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements. Acta Crystallographica. Section F, Structural biology and crystallization communications. 2011; http://hdl.handle.net/11427/21608.en_ZA
dc.languageengen_ZA
dc.publisherInternational Union of Crystallographyen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceActa Crystallographica. Section F, Structural biology and crystallization communicationsen_ZA
dc.source.urihttp://journals.iucr.org/f/index.html
dc.subject.otherglutamine synthetase III
dc.subject.otherBacteroides fragilis
dc.titleProteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangementsen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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