Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by cryo-electron microscopy

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dc.contributor.advisorSewell, Bryan Trevoren_ZA
dc.contributor.authorMiller, Jennifer Louiseen_ZA
dc.date.accessioned2014-07-30T17:40:09Z
dc.date.available2014-07-30T17:40:09Z
dc.date.issued2007en_ZA
dc.description.abstractHeterocapsa circularisquama RNA virus is a non-enveloped icosahedral ssRNA virus infectious to the harmful bloom-forming dinoflagellate, H. circularisquama, and which is assumed to be the major natural agent controlling the host population. The viral capsid is constructed from a single gene product. Electron cryo-microscopy revealed that the virus has a diameter of 34 nm and T53 symmetry. The 180 quasi-equivalent monomers have an unusual arrangement in that each monomer contributes to a ‘bump’ on the surface of the protein. Though the capsid protein probably has the classic ‘jelly roll’ b-sandwich fold, this is a new packing arrangement and is distantly related to the other positive-sense ssRNA virus capsid proteins. The handedness of the structure has been determined by a novel method involving high resolution scanning electron microscopy of the negatively stained viruses and secondary electron detection.en_ZA
dc.identifier.apacitationMiller, J. L. (2007). <i>Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by cryo-electron microscopy</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4296en_ZA
dc.identifier.chicagocitationMiller, Jennifer Louise. <i>"Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by cryo-electron microscopy."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2007. http://hdl.handle.net/11427/4296en_ZA
dc.identifier.citationMiller, J. 2007. Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by cryo-electron microscopy. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Miller, Jennifer Louise AB - Heterocapsa circularisquama RNA virus is a non-enveloped icosahedral ssRNA virus infectious to the harmful bloom-forming dinoflagellate, H. circularisquama, and which is assumed to be the major natural agent controlling the host population. The viral capsid is constructed from a single gene product. Electron cryo-microscopy revealed that the virus has a diameter of 34 nm and T53 symmetry. The 180 quasi-equivalent monomers have an unusual arrangement in that each monomer contributes to a ‘bump’ on the surface of the protein. Though the capsid protein probably has the classic ‘jelly roll’ b-sandwich fold, this is a new packing arrangement and is distantly related to the other positive-sense ssRNA virus capsid proteins. The handedness of the structure has been determined by a novel method involving high resolution scanning electron microscopy of the negatively stained viruses and secondary electron detection. DA - 2007 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2007 T1 - Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by cryo-electron microscopy TI - Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by cryo-electron microscopy UR - http://hdl.handle.net/11427/4296 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4296
dc.identifier.urihttp://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.031211-0#tab2
dc.identifier.vancouvercitationMiller JL. Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by cryo-electron microscopy. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2007 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4296en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherStructural Biologyen_ZA
dc.titleThree-dimensional reconstruction of Heterocapsa circularisquama RNA virus by cryo-electron microscopyen_ZA
dc.typeMasters Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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